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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #312339

Title: Registration of 24 ‘IR36’ rice trisomic lines

item Eizenga, Georgia
item KHUSH, GURDEV - University Of California

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/1/2015
Publication Date: 8/21/2015
Citation: Eizenga, G.C., Khush, G.S. 2015. Registration of 24 ‘IR36’ rice trisomic lines. Journal of Plant Registrations. 9:398–402. doi:10.3198/jpr2014.11.0079crgs.

Interpretive Summary: Rice is a diploid species having 12 different chromosomes, or 24 total chromosomes. Rice plants with an additional chromosome, thus three copies of a particular chromosome rather than two, have 25 total chromosomes and are called trisomic plants. In many cases, the presence of the additional chromosome changes the growth and development of the rice plant and these trisomic plants can be used in genetic mapping studies to identify the location of a particular trait or gene on a chromosome. A set of these trisomic lines was developed using the rice variety IR36 which was developed at the International Rice Research Institute in the Philippines. Twenty-four of these IR36 trisomic lines were brought through the U.S. quarantine process, re-selected based on morphology, validated by microscopic observation of the additional chromosome, and the seed stocks increased. These IR36 trisomic lines are available to the rice research community and these will be useful in mapping studies that will expedite rice genomics research and gene discovery. Seed is available through the USDA-ARS National Plant Germplasm System, Genetic Stocks-Oryza collection.

Technical Abstract: Twenty-four of the ‘IR36’ rice (Oryza sativa L.) trisomic seed stocks including ten primary trisomics lines, Triplo 1, 3, 4, 5, 7, 8, 9, 10, 11 and 12 (GSOR 101-110); seven secondary trisomic lines identified as 2n + 5S.5S, 7S.7S, 8S.8S, 8L.8L, 11S.11S, 11L.11L and 12S.12S (GSOR 111-117), seven telotrisomic lines identified as 2n + 2L, 3L, 7S, 8S, 9S, 10S and 12S (GSOR 118-124) and the disomic IR36 (GSOR 125) are being made available through the USDA ARS Genetic Stocks-Oryza (GSOR) collection. These lines were imported from the International Rice Research Institute in the Philippines and the trisomic plants were reselected as mature plants in a quarantine greenhouse and seed from the selected plants was grown in the field for several years at Stuttgart, Arkansas for seed increase and evaluation. Segregating trisomic plants were identified based on morphology and selections were confirmed by meiotic analysis. Data were collected on plant morphological (number of days to heading, plant height, panicle length, flag leaf length and width, seeds per panicle) and seed size traits from segregating trisomic plants. Rice trisomic plants can be used to locate genes on chromosomes, associate linkage groups or DNA clones to chromosome(s), and to develop chromosome specific DNA markers.