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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Avian Disease and Oncology Research » Research » Publications at this Location » Publication #311903

Title: Vaccine induced differential expressions of miRNAs at cytolytic stage in chickens resistant or susceptible to Marek’s disease

item Zhang, Huanmin
item XIE, QINGMEI - South China Agricultural University
item CHANG, SHUANG - Shandong Agricultural University
item HE, YANGHUA - University Of Maryland
item ERNST, CATHERINE - Michigan State University
item SONG, JIUZHOU - University Of Maryland

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/21/2014
Publication Date: 1/10/2015
Citation: Zhang, H., Xie, Q., Chang, S., He, Y., Ernst, C., Song, J. 2015. Vaccine induced differential expressions of miRNAs at cytolytic stage in chickens resistant or susceptible to Marek’s disease. Meeting Abstract. Plant & Animal Genome XXIII, January 10-14, 2015. Paper No. W643.

Interpretive Summary:

Technical Abstract: Gene expression regulation is critical for all cellular processes since dysregulation of it often results in elevated disease risk and compromised cellular immunity. MicroRNAs (miRNAs) directly regulate gene expression post-transcriptionally through base-pairing with regions in the 3’-untranslated sequences of the target gene mRNAs. MicroRNAs also indirectly regulate gene expression through regulation of other epigenetic factors. This study was designed to identify miRNAs differentially expressed in chickens induced by Marek’s disease (MD) vaccines. Chickens from two inbred lines (6-3 and 7-2) were included in this study. The line 6-3 is relatively resistant to MD whereas the line 7-2 is highly susceptible. Small RNA libraries were constructed with total RNAs extracted from spleen samples collected at 5 days post vaccine inoculation. Analyses of deep sequencing data resulted in 58 and 17 miRNAs differentially expressed between the HVT and non-vaccinated groups, 17 and 57 miRNAs between the CVI988/Rispens and non-vaccinated groups for line 6-3 and line 7-2, respectively (Log2 fold change > 1.5). HVT and CVI988/Rispens apparently induced strikingly differed numbers of differentially expressed miRNAs between the MD resistant and susceptible lines of chickens. The reciprocally different numbers of differentially expressed miRNAs are anticipated highly relevant to the differences of vaccine protective efficacy observed between the lines of chickens in challenge trials. Analyses for target genes of the identified miRNAs will follow. These results might lead to better understanding on what microRNAs are involved in modulating vaccine protective efficacy in chickens.