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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #311500

Research Project: SoyBase and the Legume Clade Database

Location: Corn Insects and Crop Genetics Research

Title: Genome resilience and prevalence of segmental duplications following fast neutron irradiation of soybean

Author
item Bolon, Yung-tsi - University Of Minnesota
item Stec, Adrian - University Of Minnesota
item Michno, Jean-michel - University Of Minnesota
item Roessler, Jeffrey - University Of Minnesota
item Bhaskar, Pudota - University Of Minnesota
item Ries, Landon - University Of Minnesota
item Dobbels, Austin - University Of Minnesota
item Campbell, Benjamin - University Of Minnesota
item Young, Nathan - University Of Minnesota
item Anderson, Justin - University Of Minnesota
item Grant, David
item Orf, James - University Of Minnesota
item Naeve, Seth - University Of Minnesota
item Muehlbauer, Gary - University Of Minnesota
item Vance, Carroll
item Stupar, Robert - University Of Minnesota

Submitted to: Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 9/2/2014
Publication Date: 9/10/2014
Citation: Bolon, Y., Stec, A.O., Michno, J., Roessler, J., Bhaskar, P.B., Ries, L., Dobbels, A.A., Campbell, B.W., Young, N.P., Anderson, J.E., Grant, D.M., Orf, J.H., Naeve, S.L., Muehlbauer, G.J., Vance, C.P., Stupar, R.M. 2014. Genome resilience and prevalence of segmental duplications following fast neutron irradiation of soybean. Genetics. DOI:10.1534/genetics.114.170340.

Interpretive Summary: Fast neutron irradiation was used to induce mutations in soybean. In this study, 264 mutants selected from a large mutagenized population were examined to determine the kinds and numbers of structural changes in chromosome structure that were present. Both deletions and a surprisingly high number of segmental duplications were found. Several selected mutants were analyzed in detail, and some rearrangements were found to be associated with specific phenotypes. Overall this study provides information on the numbers and kinds of structural variation induced by fast neutron irradiation and shows the utility of fast neutron-irradiated mutants as a source of novel genetic losses and gains.

Technical Abstract: Fast neutron radiation has been used as a mutagen to develop extensive mutant collections. However, the genome-wide structural consequences of fast neutron radiation are not well understood. Here, we examine the genome-wide structural variants observed among 264 soybean (Glycine max (L.) Merrill) plants sampled from a large fast neutron-mutagenized population. While deletion rates were similar to previous reports, surprisingly high rates of segmental duplication were also found throughout the genome. Duplication coverage extended across entire chromosomes and often prevailed at chromosome ends. High-throughput resequencing analysis of selected mutants resolved specific chromosomal events, including the rearrangement junctions for a large deletion, a tandem duplication, and a translocation. Genetic mapping associated a large deletion on chromosome 10 with a quantitative change in seed composition for one mutant. The tandem duplication event, located on chromosome 17 in a second mutant, was found to cosegregate with a short petiole mutant phenotype, and thus may serve as an example of a morphological change attributable to a DNA copy number gain. Overall, this study provides insight into the resilience of the soybean genome, the patterns of structural variation resulting from fast neutron mutagenesis, and the utility of fast neutron-irradiated mutants as a source of novel genetic losses and gains.