|GU, AIXING - Xinjiang Agricultural University|
|LI, DAN - Xinjiang Agricultural University|
|CHEN, QUANJIA - Xinjiang Agricultural University|
|WANG, LIPING - Xinjiang Agricultural University|
|QU, YANYING - Xinjiang Agricultural University|
Submitted to: Xinjiang Agricultural Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/16/2014
Publication Date: 3/2/2015
Citation: Gu, A., Liu, J., Li, D., Chen, Q., Wang, L., Qu, Y. 2015. Screening and evaluation of molecular markers linked with the factors affected Verticillium wilt resistance in cotton. Xinjiang Agricultural Sciences. 52:493-502.
Interpretive Summary: Verticillium wilt of cotton, often dubbed as “cancer of cotton”, has become the major impedance for continued high yield production of cotton in Xinjiang, China's largest cotton growing region. Traditional breeding has made little progress in controlling the disease in recent years. Understanding the resistance mechanism at the molecular level allows integrating the traditional breeding with biotechnology to develop new resistance cultivars. Present work developed gene markers associated with disease resistance and screened the existing gene markers for associations with disease resistance. Several markers consistently appeared in the resistant plants but not in the susceptible plants. These markers will be useful in developing resistant cultivars.
Technical Abstract: The purpose of this study is to search the consistency of the factors affecting Verticillium wilt resistance and resistant levels in cotton to further study the wilt resistant genes and genetic mechanism of resistance. Method: Thirty-seven pairs of primers derived from Verticillium wilt resistance factors were used in PCR to investigate the distribution of wilt resistant genes among resistant, tolerant, and susceptible cotton cultivars, and the consistency of selected markers with the resistance performance of cotton cultivars. Result: The results indicated that eight makers VM13'VM23' VM25' BNL0946' BNL1414' BNL1721' em6me2 and em1me5 produced polymorphic fragments. These primers originated from either disease-resistant protein'or genomic'mRNA sequence' SSR and SRAP that were associated with Verticillium wilt resistance. The consistency index of the PCR fragments appearing in the majority of resistant cultivars for the primers VM13'BNL1414 and em6me2 within the resistant cultivars were greater than 0.857,while within susceptible cultivars were lower than 0.25. The consistency index of the PCR fragments appearing in the majority of Xinjiang bred cultivars within Xinjiang bred cultivars ranged 0.364~0.727 with an average of 0.515, while in other region bred cultivars ranged 0.111~0.666 with an average of 0.419. Conclusion: The polymorphic bands amplified by the three, VM13'BNL1414 and em6me2, primer pairs serve as good molecular markers for Verticillium wilt resistance in cotton.