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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Animal Metabolism-Agricultural Chemicals Research » Research » Publications at this Location » Publication #310897

Research Project: METABOLIC FATE OF CHEMICAL AND BIOLOGICAL CONTAMINANTS

Location: Animal Metabolism-Agricultural Chemicals Research

Title: Complete NMR characterization of zilpaterol

Author
item Svendsen, Skyler
item Hakk, Heldur
item Smith, David

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/29/2014
Publication Date: 10/3/2014
Citation: Svendsen, S.A., Hakk, H., Smith, D.J. 2014. Complete NMR characterization of zilpaterol [abstract]. Undergraduate Research in the Molecular Sciences 2014. Moorhead, MN. October 3-4, 2014. Abstract No. 31.

Interpretive Summary: Zilpaterol is a ß-agonist feed additive used by cattle producers to increase the feed efficiency and weight gain of beef animals, leading to increased value. Typical applications are made during the final 3-6 weeks prior to slaughter; a short withdrawal period prior to animal slaughter is required to deplete residues in edible tissues. Because a [14C]labeled zilpaterol analog would be highly useful in metabolism and pharmacokinetic studies, our laboratory began to synthesize such a radiolabeled compound. However, our search of the chemical literature did not yield 1H- and 13C-NMR spectra needed to confirm our synthetic target. Therefore, the goal of this report is to assign all proton and carbon signals obtained from NMR spectra produced from zilpaterol isolated from the commercial feed supplement. NMR experiments performed included 1H-, 13C-, COSY, HETCOR and DEPT-135.

Technical Abstract: Zilpaterol is a ß-agonist feed additive used by cattle producers to increase the feed efficiency and weight gain of beef animals, leading to increased value. Typical applications are made during the final 3-6 weeks prior to slaughter; a short withdrawal period prior to animal slaughter is required to deplete residues in edible tissues. Because a [14C]labeled zilpaterol analog would be highly useful in metabolism and pharmacokinetic studies, our laboratory began to synthesize such a radiolabeled compound. However, our search of the chemical literature did not yield 1H- and 13C-NMR spectra needed to confirm our synthetic target. Therefore, the goal of this report is to assign all proton and carbon signals obtained from NMR spectra produced from zilpaterol isolated from the commercial feed supplement. NMR experiments performed included 1H-, 13C-, COSY, HETCOR and DEPT-135.