Submitted to: North Agricultural Biotechnology Council (NABC) — New DNA-Editing Approaches: Methods, Applications and Policy for Agriculture
Publication Type: Proceedings
Publication Acceptance Date: 3/14/2015
Publication Date: 6/1/2015
Citation: Blomberg, L. 2015. Potential for selection of beneficial traits in swine with site-specific sucleases. North Agricultural Biotechnology Council (NABC) — New DNA-Editing Approaches: Methods, Applications and Policy for Agriculture. 26:125-133.
Technical Abstract: Selective breeding in agricultural species has enabled the derivation of stronger and fitter animals with improved production traits. However, along with beneficial traits there is often the co-segregation of less desirable traits. With the plethora of genome data and annotation, and greater understanding of gene function and their role in specific biological mechanisms regulating growth, disease susceptibility, etc., the ability to specifically target an allele, which places an animal at risk, for silencing or repair without the introduction of foreign DNA is now being realized. Collaboratively with a university partner, clustered regularly interspaced short palindromic repeats (CRIPSR) technology with a reporter gene was utilized to directly modify a target gene (prion) in the one cell porcine embryo using both nonhomologous end joining (NHEJ) and homology-directed repair (HDR). One cell embryos were microinjected with the respective NHEJ or HDR construct and culture through the blastocyst stage was achieved. Both single (prion) and double (prion/ zinc finger, BED-type containing 6) gene knockouts were successfully generated with the NHEJ platform whereas embryos the knockins were created with HDR. Embryo transfer to recipients for the derivation of genome-edited off-spring is currently ongoing. The goal will be to utilize these technologies, in concert with industry, to address disease and animal-welfare issues.