|Kuhl, Joseph - University Of Idaho|
|Novy, Richard - Rich|
|Dibble, Margaret - University Of Idaho|
Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/28/2015
Publication Date: 6/22/2016
Citation: Kuhl, J.C., Novy, R.G., Whitworth, J.L., Dibble, M., Schneider, B.L., Hall, D.G. 2016. Development of molecular markers closely linked to the potato leafroll virus resistance gene, Rlretb, for use in marker-assisted selection. American Journal of Potato Research. 93(3):203-212.
Interpretive Summary: Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. A gene for resistance to PLRV, Rlretb, has been used in breeding. The development of molecular markers tightly linked to this gene would facilitate the development of PLRV-resistant potato varieties. Molecular markers in very close proximity to Rlretb were developed which will be useful to potato breeders for maker-assisted selection for PLRV.
Technical Abstract: Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary varieties grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum- derived dominant gene Rlretb confers resistance to potato leafroll virus and has been mapped to chromosome 4. The previous closest marker to Rlretb , C2_At1g42990, was mapped 13.6 cM from the gene in a BC3 population. The development of additional molecular markers closely linked to Rlretb can significantly facilitate its use in breeding and its continued introgression into cultivated potato. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlretb in 102 BC4 progeny. Sixteen markers were identified surrounding Rlretb, with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlretb, are shown to be well suited for marker assisted selection. Currently, breeding clones with the presence of Rlretb have been developed that comprise market classes characterized by having tubers that are round and white, or long and russet-skinned.