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United States Department of Agriculture

Agricultural Research Service

Research Project: DISCOVERY AND UTILIZATION OF BIOACTIVE COMPONENTS FROM NEW CROPS AND AGRICULTURAL CO-PRODUCTS

Location: Functional Foods Research

Title: Collision-induced fragmentation accurate mass spectrometric analysis methods to rapidly characterize plant extracts

Author
item Berhow, Mark
item Bowman, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/4/2014
Publication Date: 10/4/2014
Citation: Berhow, M.A., Bowman, M.J. 2014. Collision-induced fragmentation accurate mass spectrometric analysis methods to rapidly characterize plant extracts [abstract]. Illinois Heartland Local Section of the American Chemical Society.

Interpretive Summary:

Technical Abstract: The rapid advances in analytical chromatography equipment have made the reliable and reproducible measurement of a wide range of plant chemical components possible. Full chemical characterization of a given plant material is possible with the new mass spectrometers currently available. For phytochemicals, we have used the latest accurate mass LC-MS analysis to quickly characterize the potential chemical formulas for a series of unknown compounds in a variety of plant extracts using a standardized CID/HCD mass fragmentation experiment in which interpretation of daughter ions produced allowed for the identification of a variety of phytochemical constituents. These results – coupled with DEPT NMR analysis as needed – resulted in the identification of a series of phytochemicals in camelina and sorghum pith extracts. Accurate LC-MSN screening and characterization analyses can be coupled to more rapid non-destructive spectrophotometric analytical methods such as pulsed NMR and near infrared (NIR) spectrometry, which will allow for the rapid and non-destructive analysis of thousands of samples for a wide range of chemical composition parameters.

Last Modified: 10/19/2017
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