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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #309986

Research Project: Discovery and Introgression of Disease Resistance Genes into Phaseolus vulgaris

Location: Soybean Genomics & Improvement Laboratory

Title: Registration of PR0806-80 and PR0806-81 white bean germplasm lines with resistance to BGYMV, BCMV, BCMNV and rust

Author
item Beaver, James - University Of Puerto Rico
item Rosas, J - Escuela Agricola Panamericana
item Porch, Timothy - Tim
item Pastor Corrales, Marcial - Talo
item Godoy-lutz, G - Dominican Institute For Agricultural And Forestry Research (IDIAF)
item Prophete, E - Ministry Of Agriculture-Haiti

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/12/2014
Publication Date: 2/6/2015
Citation: Beaver, J.S., Rosas, J.C., Porch Clay, T.G., Pastor Corrales, M.A., Godoy-Lutz, G., Prophete, E.H. 2015. Registration of PR0806-80 and PR0806-81 white bean germplasm lines with resistance to BGYMV, BCMV, BCMNV and rust. Journal of Plant Registrations. 9(2):208-211.

Interpretive Summary: Diseases are major constraints to common bean production in both tropical and temperate bean production regions. The rust disease, as well as bean common mosaic necrosis virus (BCMNV) and bean common mosaic virus (BCMV), caused by seed-borne potyviruses, and Bean golden yellow mosaic virus (BGYMV), a whitefly-transmitted Begomovirus, reduce yields and are major threats to common bean production in Central America and the Caribbean. PR0806-80 and PR0806-81 are multiple disease resistant dry bean germplasm lines, adapted to the humid tropics that were developed and released cooperatively by scientists from the University of Puerto Rico, ARS-USDA, Zamorano University in Honduras, and the Departments of Agriculture of the Dominican Republic and Haiti. These lines combine three genes that confer broad resistance to all known strains of the rust pathogen with two genes for resistance to all known strains of BCMV and BCMNV and two genes for resistance to BGYMV. The lines were evaluated as resistant to many strains of the rust pathogen under greenhouse conditions at ARS-USDA, Maryland, and under field conditions in Puerto Rico and Honduras. Both lines were also evaluated in field trials planted over a period of three years in the Dominican Republic, Haiti and Puerto Rico. Their mean seed yields were comparable with the best check cultivar. These multiple disease resistant white seeded lines should be useful as parents to enhance the virus and rust resistance of white, small red and black beans produced in Central America and the Caribbean.

Technical Abstract: PR0806-80 (Reg. No. GP-___, PI ______) and PR0806-81 (Reg. No. GP-___, PI ______) are multiple disease resistant dry bean (Phaseolus vulgaris L.) germplasm lines, adapted to the humid tropics, that were developed and released cooperatively by the University of Puerto Rico Agricultural Experiment Station, the USDA-ARS, the Escuela Agrícola Panamericana (Zamorano), the Instituto Dominicano de Investigaciones Agropecuarias y Forestales and the Ministry of Agriculture, Natural Resources and Rural Development of the Republic of Haiti. The breeding objective was to develop white dry bean lines that combine resistance to Bean common mosaic virus (BCMV), Bean common mosaic necrotic virus BCMNV), Bean golden yellow mosaic virus (BGYMV) and rust, caused by Uromyces appendiculatus. PR0806-80 and PR0806-81 were developed by pedigree selection to the F6 generation based on superior agronomic traits and commercial seed type. Advanced generation lines were screened for rust resistance in Honduras and were resistant to BGYMV, BCMNV and rust in trials planted in Puerto Rico, the Dominican Republic and Haiti. Results from greenhouse inoculations with specific strains of bean rust at USDA-ARS-Beltsville suggest that the lines combine the Ur-4, Ur -5, and Ur-11 rust resistance genes. Greenhouse inoculations were used to detect the presence of the bc-3 gene for resistance to BCMV and BCMNV, and marker-assisted selection was used to identify the presence of the bgm-1 gene and the SW12 QTL for BGYMV resistance. The presence of SA14 and SI19 SCAR markers provided further evidence that these lines have the rust resistance genes Ur-4 and Ur-5. The lines were evaluated in field trials planted over a period of three years in the Dominican Republic, Haiti and Puerto Rico. Mean seed yields of PR0806-80 (2,048 kg ha-1) and PR0806-81 (2,091 kg ha-1) were comparable with the check cultivar ‘Verano ' (2,251 kg ha-1). These multiple disease resistant white lines should be useful as parents to enhance the virus and rust resistance of white, small red and black beans.