|BHARDWAJ, HARBANS - Virginia State University|
Submitted to: Food Legume Research International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/30/2014
Publication Date: 7/1/2014
Citation: Kisha, T.J., Bhardwaj, H.L. 2014. Genetic diversity of the Phaseolus acutifolius A. Gray collection of the USDA National Plant Germplasm system using targeted region amplified polymorphism markers designed from genes associated with heat and drought stress [abstract]. Food Legume Research International Conference Proceedings. Conference Abstract Book.
Technical Abstract: Molecular genetic relationships among 222 accessions of the Phaseolus acutifolius A. Gray collection were assessed using Targeted Region Amplified Polymorphic (TRAP) markers designed from sequences of genes associated with heat and drought tolerance. Genetic relationships were compared to reactions to drought stress using measurements of variable fluorescence to maximum fluorescence (Fv/Fm). Cluster analysis using NTSys-pc and STRUCTURE found 3 major groups and 8 sub-groups with an average Fst of o.71, but there was no association of drought tolerance with any group. In fact, some plants separated by seed color within an accession tested as both drought tolerant and susceptible, though genetically similar at all marker loci tested. In other cases, plants separated by seed type clustered apart in different groups. Given the level of diversity among groups, marker production will continue along with the addition of AFLP loci for future association mapping.