Skip to main content
ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #309467

Research Project: Development and Characterization of Soybean Germplasm, Curation of Stored Accessions, and Regional Evaluations of New Genotypes

Location: Crop Genetics Research

Title: Screening a diverse soybean germplasm collection for reaction to purple seed stain caused by Cercospora kikuchii

Author
item ALLOATTI, JULIETA - University Of Arkansas
item Li, Shuxian
item CHEN, PENGYIN - University Of Arkansas
item JAUREGUY, LUCIANO - University Of Arkansas
item SMITH, S - University Of Arkansas
item FLOREZ-PALACIOU, LILIANA - University Of Arkansas
item ORAZALY, MOLDIR - University Of Arkansas
item RUPE, JOHN - University Of Arkansas

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2015
Publication Date: 8/7/2015
Citation: Alloatti, J., Li, S., Chen, P., Jaureguy, L., Smith, S.F., Florez-Palaciou, L., Orazaly, M., Rupe, J. 2015. Screening a diverse soybean germplasm collection for reaction to purple seed stain caused by Cercospora kikuchii. Plant Disease. 99:1140-1146.

Interpretive Summary: Purple seed stain (PSS), is a prevalent soybean disease that causes latent seed infection, seed decay, purple seed discoloration, and overall quality deterioration. The objective of this research was to screen soybean accessions from the USDA germplasm collection for resistance to PSS. A total of 123 plant introductions (PI) from 28 different countries, representing maturity groups (MG) III, IV, and V, were screened. Percentage of field Cercospora leaf blight (% CLB) and percentages of visual PSS (% PSS) and percentages of seed infected by Cercospora kikuchii (% C. kikuchii, the fungus causing PSS and CLB) in harvested seed were determined. Overall, % C. kikuchii for inoculated plots in 2007 ranged from 2 to 51% for MG III, 2 to 35% for MG IV, and 0 to 33% for MG V. A total of 5 and 11 PIs from MG III and IV, respectively, were identified as resistant soybean for PSS. The PSS resistant PIs identified in this study will be valuable to breeders in developing resistant cultivars.

Technical Abstract: Purple seed stain (PSS), caused by Cercospora kikuchii, is a prevalent soybean disease that causes latent seed infection, seed decay, purple seed discoloration, and overall quality deterioration. The objective of this research was to screen soybean accessions from the USDA germplasm collection for resistance to PSS. A total of 123 plant introductions (PI) from 28 different countries, representing maturity groups (MG) III, IV, and V, were screened. Percentage of field Cercospora leaf blight (% CLB) and percentages of visual PSS (% PSS) and percentages of seed infected by C. kikuchii (% C. kikuchii) in harvested seed were determined. Overall, % C. kikuchii for inoculated plots in 2007 ranged from 2 to 51% for MG III, 2 to 35% for MG IV, and 0 to 33% for MG V. A total of 5 and 11 PIs from MG III and IV, respectively, were identified as resistant genotypes for PSS. Highly positive correlations were found for inoculated vs. non-inoculated treatments and for % PSS vs. % C. kikuchii infection. The PSS resistant PIs identified in this study will be valuable to breeders in developing resistant cultivars.