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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #309445

Research Project: Enhancement of Wheat through Genomic and Molecular Approaches

Location: Crop Improvement and Genetics Research

Title: Registration of isogenic HMW-GS Ax1 protein deficient lines of an elite wheat variety

Author
item L Chingcuanco, Debbie

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/2014
Publication Date: 4/24/2015
Citation: Chingcuanco, D.L. 2015. Registration of isogenic HMW-GS Ax1 protein deficient lines of an elite wheat variety. Journal of Plant Registrations. doi: 10.3198/jpr2014.09.0064crgs.

Interpretive Summary: The high molecular weight glutenin subunits (HMW-GS) of wheat gluten are the major determinants of dough quality. Bread wheat has six sets of HMW-GS genes and variations in the HMW-GS genes have been correlated with the differences in dough quality in wheat varieties. In this work, we report the creation, identification and preliminary characterization of a set of novel isogenic wheat lines with different composition and dosages of HMW-GS protein in one of the wheat gene sets. This set is a useful tool to determine the role of these proteins in dough properties. Here we register and report the identification of several Ax1 protein deficient lines from mutagenized populations of the elite bread wheat variety Triticum aestivum cv. Summit.

Technical Abstract: A series of isogenic wheat lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded on the A-genome of an elite variety of bread wheat (Triticum aestivum L.) was developed by USDA-ARS at the Western Regional Research Center in Albany, CA. This set of A-genome encoded HMW-GS deficient lines was identified from chemical and radiation-mutagenized population of hexaploid wheat variety Summit by screening M3 seeds for altered storage protein profiles using one-dimensional SDS-PAGE. The mutant lines are distinct from the wild-type progenitor by the absence of the HMW-GS Ax1 protein. Dough derived from greenhouse and field grown plants of one of the mutants exhibited weaker dough but achieved shorter development time compared to that of the control. There are six HMW-GS loci in bread wheat and each locus vary in their effect on dough quality. Isogenic wheat lines deficient in the individual HMW-GS will allow the determination of the contributions of the different alleles in each locus to dough quality.