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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Quality & Safety Assessment Research » Research » Publications at this Location » Publication #308621

Research Project: Optical Detection of Food Safety and Food Defense Hazards

Location: Quality & Safety Assessment Research

Title: Foodborne pathogen detection

item Yoon, Seung-Chul

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 2/27/2014
Publication Date: 10/15/2015
Citation: Yoon, S.C. 2015. Foodborne pathogen detection using hyperspectral imaging. In Park and Lu (eds.) Hyperspectral Imaging Technology in Food and Agriculture, Springer, Book Chapter. Chapter 7: pp. 173-201.

Interpretive Summary: none

Technical Abstract: Foodborne pathogens can cause various diseases and even death when humans consume foods contaminated with microbial pathogens. Traditional culture-based direct plating methods are still the “gold standard” for presumptive-positive pathogen screening. Although considerable research has been devoted to development and use of biochemical, serological, and molecular methods for confirmation of presumptive-positive colonies, such as latex agglutination and polymerase chain reaction (PCR), little research has been done to develop methods and techniques for non-invasive optical screening of presumptive-positive colonies, while keeping them on agar-filled Petri dishes. Hyperspectral imaging is a non-destructive and non-contact optical imaging technique that combines aspects of conventional imaging and vibrational spectroscopy so that data can provide two-dimensional spatial information on colony shapes and spectral information at every pixel in each colony under test. The spectral “fingerprints” of bacteria provided by hyperspectral imaging can be used for detection and identification of pathogens. This book chapter covers the potential and efficacy of hyperspectral imaging for detection and identification of pathogenic colonies such as Campylobacter and Shiga-toxin producing Escherichia coli (STEC).