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United States Department of Agriculture

Agricultural Research Service

Research Project: Management of Genetic Resources & Associated Information for Grape, Tree Fruit, Tree Nut, & Other Specialty Crops to Mediterranean Climates

Location: Nat'l Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes

Title: A modern amplelography: A genetic basis for leaf shape and venation patterning in grape

Author
item Chitwood, Daniel
item Ranjan, Asahish
item Martinez, Ciera
item Headland, Lauren
item Thiem, Thinh
item Kumar, Ravi
item Covington, Michael
item Hatcher, Tommy
item Naylor, Daniel
item Zimmerman, Sharon
item Downs, Nora
item Raymundo, Nataly
item Buckler, Edward
item Maloof, Julian
item Aradhya, Mallikarjuna
item Prins, Bernard - Bernie
item Li, Lin
item Myles, Sean
item Sinha, Neelima

Submitted to: Plant Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/25/2013
Publication Date: 11/27/2013
Citation: Chitwood, D.H., Ranjan, A., Martinez, C.C., Headland, L.R., Thiem, T., Kumar, R., Covington, M.E., Hatcher, T., Naylor, D.T., Zimmerman, S., Downs, N., Raymundo, N., Buckler, E.S., Maloof, J.N., Aradhya, M.K., Prins, B.H., Li, L., Myles, S., Sinha, N.R. 2013. A modern amplelography: A genetic basis for leaf shape and venation patterning in grape. Plant Physiology. 164:259-272..

Interpretive Summary: Terroir, the unique interaction between genotype, environment, and culture, is highly refined in domesticated grape (Vitis vinifera). Toward cultivating terroir, the science of ampelography tried to distinguish thousands of grape cultivars without the aid of genetics. This led to sophisticated phenotypic analyses of natural variation in grape leaves, which within a palmate-lobed framework exhibit diverse patterns of blade outgrowth, hirsuteness, and venation patterning. Here, we provide a morphometric analysis of more than 1,200 grape accessions. Elliptical Fourier descriptors provide a global analysis of leaf outlines and lobe positioning, while a Procrustes analysis quantitatively describes venation patterning. Correlation with previous ampelography suggests an important genetic component, which we confirm with estimates of heritability. We further use RNA-Seq of mutant varieties and performa genome-wide association study to explore the genetic basis of leaf shape. Meta-analysis reveals a relationship between leaf morphology and hirsuteness, traits known to correlate with climate in the fossil record and extant species. Together, our data demonstrate a genetic basis for the intricate diversity present in grape leaves. We discuss the possibility of using grape leaves as a breeding target to preserve terroir in the face of anticipated climate change, a major problem facing viticulture.

Technical Abstract: Terroir, the unique interaction between genotype, environment, and culture, is highly refined in domesticated grape (Vitis vinifera). Toward cultivating terroir, the science of ampelography tried to distinguish thousands of grape cultivars without the aid of genetics. This led to sophisticated phenotypic analyses of natural variation in grape leaves, which within a palmate-lobed framework exhibit diverse patterns of blade outgrowth, hirsuteness, and venation patterning. Here, we provide a morphometric analysis of more than 1,200 grape accessions. Elliptical Fourier descriptors provide a global analysis of leaf outlines and lobe positioning, while a Procrustes analysis quantitatively describes venation patterning. Correlation with previous ampelography suggests an important genetic component, which we confirm with estimates of heritability. We further use RNA-Seq of mutant varieties and performa genome-wide association study to explore the genetic basis of leaf shape. Meta-analysis reveals a relationship between leaf morphology and hirsuteness, traits known to correlate with climate in the fossil record and extant species. Together, our data demonstrate a genetic basis for the intricate diversity present in grape leaves. We discuss the possibility of using grape leaves as a breeding target to preserve terroir in the face of anticipated climate change, a major problem facing viticulture.

Last Modified: 06/21/2017
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