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United States Department of Agriculture

Agricultural Research Service

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus)

Author
item Zhen, W.
item Huang, Lili
item Huang, Jinqun
item Wang, Xiaojie
item Chen, Xianming
item Zhao, Jie
item Guo, Jun
item Zhuang, Hua
item Qiu, Chuangzhao
item Liu, Jie
item Liu, Huiquan
item Huang, Xueling
item Pei, Guoliang
item Zhan, Gangming
item Tang, Chunlei
item Cheng, Yulin
item Liu, Minjie
item Zhang, Jinshan
item Zhao, Zhongtao
item Zhang, Shijie
item Han, Qingmei
item Han, Dejun
item Zhang, Hongzhang
item Zhao, Jing
item Gao, Xiaoning
item Wang, Jianfeng
item Ni, Peixiang
item Dong, Wei
item Yang, Linfeng
item Yang, Huanming
item Xu, Jinrong
item Zhang, Gengyun
item Kang, Zhenshen

Submitted to: Nature Communications
Publication Type: Peer reviewed journal
Publication Acceptance Date: 9/26/2013
Publication Date: 10/23/2013
Publication URL: http://www.nature.com/ncomms/2013/131023/ncomms3673/full/ncomms3673.html
Citation: 2013. High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus. Nature Communications. 4:2673 (1-10).

Interpretive Summary: Stripe rust is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of stripe rust pathogen isolate CY32, obtained using a ‘fosmid-to-fosmid’ strategy, to better understand its race evolution and pathogenesis. The fungal pathogen genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, the pathogen has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of single nucleotide polymorphisms (SNPs) are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in the pathogen suggests that sexual reproduction may have a role in the origin of different regional races. Our results show the effectiveness of the ‘fosmid-to-fosmid’ strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in the stripe rust pathogen and other obligate pathogens.

Technical Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a ‘fosmid-to-fosmid’ strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the ‘fosmid-to-fosmid’ strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.

Last Modified: 8/24/2016
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