Author
YANG, LIMING - University Of Georgia | |
JIANG, TINGBO - Northeast Forestry University | |
Scully, Brian | |
LEE, DEWEY - University Of Georgia | |
KEMERAIT, ROBERT - University Of Georgia | |
SIXUE, CHEN - University Of Florida | |
Guo, Baozhu |
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 7/3/2014 Publication Date: 8/8/2014 Citation: Yang, L., Jiang, T., Scully, B.T., Lee, D., Kemerait, R., Sixue, C., Guo, B. 2014. Drought-responsive protein profiles reveal diverse defense pathways in corn kernels under field drought atress. Meeting Abstract. American Phytopathological Society Abstracts. American Phytopathological Society meeting, August 9-13, 2014, Minneapolis, Minnesota. Interpretive Summary: Technical Abstract: Drought stress is a major factor which contributes to disease susceptibility and yield loss in agricultural crops. To identify drought responsive proteins and explore metabolic pathways involved in maize tolerance to drought stress, two lines (B73 and Lo964) with contrasting drought sensitivity were examined and the treatments of drought and well-watered were applied at 14 days after pollination (DAP), and protein profiles were investigated in developing kernels (35 DAP) using iTRAQ (isobaric tags for relative and absolute quantitation). Proteomic analysis showed that 70 and 36 proteins were significantly altered expression under drought treatments in B73 and Lo964, respectively. The numbers and levels of differentially expressed proteins were generally higher in the sensitive genotype B73, implying an increased sensitivity to drought given the function of the observed differentially expressed proteins such as redox homeostasis, cell rescue/defense, hormone regulation, protein biosynthesis and degradation. Lo964 possessed more stable status with fewer differentially expressed proteins. However, B73 seems to rapidly initiate signaling pathways in response to drought through adjusting diverse defense pathways. These changes in protein expression allow for the production |