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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #306563

Research Project: Genetic Improvement of Cool Season Food Legumes

Location: Grain Legume Genetics Physiology Research

Title: A validated source panel of SSR markers for effective discrimination of lentil species

Author
item Sen Gupta, Debjyoti - NORTH DAKOTA STATE UNIVERSITY
item Cheng, Peng - WASHINGTON STATE UNIVERSITY
item Sablok, Gaurav - FONDAZIONE EDMUND MACH
item Thavarajah, Dil - NORTH DAKOTA STATE UNIVERSITY
item Thavarajah, Pushparajah - NORTH DAKOTA STATE UNIVERSITY
item Mcgee, Rebecca
item Coyne, Clarice - Clare
item Main, Dorrie - WASHINGTON STATE UNIVERSITY
item Kumar, Shiv - INTERNATIONAL CENTER FOR AGRICULTURAL RESEARCH IN THE DRY AREAS (ICARDA)

Submitted to: Food Legume Research International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/2/2014
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Lentil (Lens culinaris Medic) is an important cool season food legume grown extensively in the world. This crop species has 14 chromosomes (2n=14), is a diploid and has a genome size of more than 4000 Mbp. Development of highly polymorphic molecular markers is a lentil research priority. In this study, 9,513 expressed sequence tags (EST) were systematically downloaded from the National Center for Biotechnology Information database to develop unigene-based simple sequence repeat (SSR) markers. The ESTs were assembled into 4,053 unigenes, and then analyzed to detect 374 SSRs using microsatellite identification tool, MISA. Among the 374 SSRs, 26 compound SSRs were observed. Primer pairs for these SSRs were designed using Primer3 v1.14. To classify the functional annotation of ESTs, and EST-SSRs, BLASTx searches (E-value, 1x10-5) were performed against the publicly available UniProt (www.uniprot.org) and NCBI (www.ncbi.nlh.nih.gov) databases. Further functional annotation was performed using the PLAZA comparative genomics and GO annotation was slimmed using the Plant GO Slim category. Among the synthesized 312 primers, 219 successfully amplified Lens DNA. A diverse panel of twenty four Lens genotypes consisting of L. culinaris advanced breeding lines, parents of mapping populations, wild types and genotypes of L. nigricans, L. culinaris ssp. orientalis, L. lamottei were tested to identify polymorphic markers. A highly polymorphic set of 57 markers successfully discriminated the test genotypes. This set of polymorphic markers with the functional annotation data could be used as molecular tools to lentil breeding.