|CAMUS, MELINDA - University Of Georgia|
|CAMUS, ALVIN - University Of Georgia|
|Leeds, Timothy - Tim|
|Wiens, Gregory - Greg|
Submitted to: International Aquatic Animal Health Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2014
Publication Date: 9/1/2014
Citation: Marancik, D.P., Camus, M.S., Camus, A.C., Leeds, T.D., Gao, G., Wiens, G.D. 2014. Exploring mechanisms of survival in rainbow trout selectively bred for increased resistance to Flavobacterium psychrophilum [abstract]. International Aquatic Animal Health Symposium Proceedings. Paper No. 102.
Technical Abstract: A challenge for selective breeding programs is to better understand how artificial selection alters host pathophysiologic and immunologic response following pathogen exposure. The National Center for Cool and Cold Water Aquaculture is exploring this in rainbow trout bred for increased survival (ARS-Fp-R line) and susceptibility (ARS-Fp-S line) to Flavobacterium psychrophilum by comparing responses within the context of host “resistance” and “tolerance”. Resistance and tolerance are assessed by quantifying the level at which host health is affected by infection intensity. Surrogate measures of host health were developed in ARS-Fp-R and ARS-Fp-S line fish by measuring packed cell volume (PCV) and plasma biochemistry analytes and correlating changes in analyte levels with qPCR-derived splenic bacterial loads. Results suggest that selective breeding has increased host resistance with little change in tolerance in the ARS-Fp-R line. Current efforts to elucidate mechanisms of resistance include proteomic profiling of plasma of ARS-Fp-R and ARS-Fp-S line fish following experimental challenge. Changes in plasma protein levels were analyzed at six days post-infection using iTRAQ and 2D-LC-MS/MS analysis and searching resultant peptide fragment sequences against the published rainbow trout genome (https://www.genoscope.cns.fr/trout). Proteins demonstrating significant differential abundance over time and between genetic lines are being categorized through bioinformatic analyses. We anticipate that identified immunologic and physiologic pathway differences between ARS-Fp-R and ARS-Fp-S line fish will reveal how selective breeding has altered disease pathogenesis and host survival.