Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: BIOLOGY OF OBESITY PREVENTION

Location: Healthy Body Weight Research

Title: Eicosapentaenoic acid regulates brown adipose tissue gene expression and metabolism in high fat fed mice)

Author
item Pahlavani, Mandanna
item Kalupahana, Nishan
item Lemieux, Monique
item Aljawadi, Arwa
item Scoggin, Shane
item Claycombe, Kate
item Moustaid-moussal, Naima

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2014
Publication Date: 4/30/2014
Citation: Pahlavani, M., Kalupahana, N.S., Lemieux, M., Aljawadi, A., Scoggin, S., Claycombe, K.J., Moustaid-Moussal, N. 2014. Eicosapentaenoic acid regulates brown adipose tissue gene expression and metabolism in high fat fed mice. Federation of American Societies for Experimental Biology Conference. 28:1037.5.

Interpretive Summary:

Technical Abstract: Brown adipose tissue (BAT) is a thermogenic tissue, a key regulator of energy balance and a potential therapeutic target for obesity. We previously reported that eicosapentaenoic acid (EPA) reduced high fat (HF) diet-induced obesity and insulin resistance in mice, independent of energy intake. We hypothesized that these effects are mediated in part by BAT thermogenesis. Using mice fed HF or HF-EPA diets for 11 weeks, we demonstrated that BAT from HF-EPA mice expressed higher mRNA levels of thermogenic genes such as fibronectin type lll domain containing 5 (FNDC5), peroxisome proliferator activated receptor gamma coactivator 1 alpha (PGC1a) and uncoupling protein 3 (UCP3), compared to HF mice. EPA also induced expression of angiotensinogen (Agt) gene and other genes related to insulin sensitivity such as Glut 1 while downregulating arachidonate 5-lipoxygenase (ALOX5), an inflammatory biomarker. At the protein level, EPA upregulated uncoupling protein (UCP) and downregulated fatty acid synthase (FAS). Thus, EPA exerts dual effects on adipose tissue depots, by reducing WAT inflammation and lipid accumulation, while activating BAT thermogenesis and reducing lipogenesis. In conclusion, EPA exerts differential tissue specific effects to reduce obesity-associated metabolic disorders. Further molecular studies in cultured brown adipocytes are being conducted to dissect direct effects of EPA on brown fat.

Last Modified: 8/24/2016
Footer Content Back to Top of Page