|RONG, XIAOYING - Chinese Academy Of Sciences|
|HUANG, YING - Chinese Academy Of Sciences|
|DOROGHAZI, JAMES - University Of Illinois|
|JU, KU-SAN - University Of Illinois|
|METCALF, WILLIAM - University Of Illinois|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2014
Publication Date: 8/1/2014
Citation: Labeda, D.P., Rong, X., Huang, Y., Doroghazi, J.R., Ju, K., Metcalf, W.W. 2014. Taxonomic evaluation of Streptomyces hirsutus and related species using multi-locus sequence analysis [abstract]. International Congress of Bacteriology and Applied Microbiology.
Technical Abstract: Phylogenetic analyses of species of Streptomyces based on 16S rRNA gene sequences resulted in a statistically well-supported clade (100% bootstrap value) containing 8 species having very similar gross morphology. These species, including Streptomyces bambergiensis, Streptomyces chlorus, Streptomyces cyanoalbus, Streptomyces emeiensis, Streptomyces hirsutus, Streptomyces prasinopilosus, Streptomyces prasinus, and Streptomyces viridis, all produce open looped (Retinaculum-Apertum) to spiral (Spira) chains of spiny to hairy surfaced, dark green spores on the aerial mycelium. These strains were subjected to multilocus sequence analysis (MLSA) utilizing partial sequences of the house-keeping genes atpD, gyrB, recA, rpoB, and trpB to clarify their taxonomic status. Phylogenetic trees were constructed using three different algorithms using alignments resulting from head to tail concatenation of the house-keeping loci. Multilocus Sequence Analysis (MLSA) distances (Kimura 2-parameter distance) were calculated between the species pairs in this clade to determine if any were related at the species level (i.e., MLSA distance < 0.007). The MLSA data suggest that several pairs of the species under study are likely be con-specific, more specifically: S. cyanoalbus NRRL B-3040T represents a later synonym of S. hirsutus NRRL B-2173T (MLSA distance is 0.002) with identical alleles of 3 loci and only 2 bp differences over the 2519 bp alignment; S. bambergiensis NRRL B-12521T is a probable later synonym of S. prasinus NRRL B-2712T (MLSA distance is 0.003) with identical alleles for two loci and 7 bp differences over the 2519 bp alignment; S. eimeiensis NRRL B-24621T is a probable later synonym of S. prasinopilous NRRL B-2711T (MLSA distance is 0.003) with identical alleles of the rpoB locus and 7 bp differences over the 2519 bp alignment, predominantly in the recA locus. Streptomyces chlorus KACC 20902T and Streptomyces viridis KACC 21003T were observed to exhibit MLSA distances of > 0.007 to the other strains studied and therefore represent unique species.