Location: National Clonal Germplasm RepositoryTitle: Effect of pretreatment methods of dormant pear buds on viability after cryopreservation Author
Submitted to: World Applied Sciences Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2014
Publication Date: 2/9/2014
Citation: Zhumagulova, Z.B., Kovalchuk, I., Reed, B.M., Turdiev, T. 2014. Effect of pretreatment methods of dormant pear buds on viability after cryopreservation. World Applied Sciences Journal. 30(3):330-334. DOI: 10.5829/idosi.wasj.2014.30.03.14028. Interpretive Summary: Storing dormant plant material by drying and immersion in liquid nitrogen is used for many cold hardy plants. Pears do not readily store using this method. This study explored changes in the protocol by using cryoprotectants during the procedure to improve the viability of pear dormant buds. We used six chemical combinations on four local Kazakhstan pear cultivars. Two moisture subgroups were tested: natural moisture of the buds (field moisture) and buds dried to 30% moisture. To determine viability of bud tissues we conducted microscopic analysis and also used staining for viability. The application of these solutions to dormant buds showed that one chemical solution (PVS3) gave much better survival of the dormant buds after cryopreservation than the others.
Technical Abstract: This study aimed to develop alternatives for dormant bud cryopreservation by using several cryoprotectants on four pear cultivars with a view to improve the viability of the dormant buds. We used different cryoprotectants such as Honey, PVS2, PVS3, PVS4, Towill, IPBB-1 for cultivars: Talgarskaya Krasavitca, Nagima, Zhazdyk, Lesnaya Krasavitca. The buds were divided into two moisture control subgroups: natural moisture of the buds (field moisture) and buds dried to 30% moisture. To determine viability of bud tissues we conducted histological analysis and triphenyltetrazolium chloride (TTC) staining. The application of these solutions to cryoprotectants viability of dormant buds showed that the PVS3 results were better than those of the other cryoprotectants.