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Title: Progress toward the development of recombinant inbred lines in potato

Author
item Jansky, Shelley
item DOUCHES, DAVID - Michigan State University
item ENDELMAN, JEFF - University Of Wisconsin

Submitted to: American Journal of Potato Research
Publication Type: Proceedings
Publication Acceptance Date: 3/27/2014
Publication Date: 7/27/2014
Citation: Jansky, S.H., Douches, D., Endelman, J. 2014. Progress toward the development of recombinant inbred lines in potato. American Journal of Potato Research. Paper No. G74.

Interpretive Summary:

Technical Abstract: Complexities due to tetraploid genetics limit breeding progress in potato. Diploids offer more simple genetics. Homozygous populations such as recombinant inbred lines are powerful resources for genetic mapping and the subsequent development of markers for marker-assisted selection. Most potato diploids are self-incompatible, but through the use of the dominant self-incompatibility inhibitor, Sli, we can create diploid inbred lines. We are developing sets of recombinant inbred lines (RILs) carrying genes from wild and cultivated species for improvement of tuber quality and disease resistance. Early generations (F2 and F3) are morphologically highly variable and exhibit severe inbreeding depression. Later generations (F4 and F5) are more uniform and vigorous. We are monitoring the phenotypic and genotypic effects of self-pollination to the F6 generation. Our goal is to create 100 F6 inbred lines of each of six populations. These will be shared with the research community for phenotyping and mapping.