Submitted to: American Peanut Research and Education Society Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2014
Publication Date: 1/27/2015
Citation: Bennett, R.S., Chamberlin, K.D. 2015. Greenhouse-based inoculation methods for Sclerotinia blight resistance in peanut [abstract]. In: Proceedings of the American Peanut Research and Education Society Proceedings, July 8-10, 2014, San Antonio, TX. 46:32.
Technical Abstract: Greenhouse-based assays for screening germplasm for resistance to Sclerotinia blight in peanuts can be conducted year-round, and thus may accelerate progress in breeding for resistant plants. Several techniques for assaying Sclerotinia blight resistance in the greenhouse have been proposed including methods using intact plants and detached plant parts. We compared three inoculation methods: cut petiole on intact plants, cut petiole on detached main stems partially submersed in Hoagland's solution, and detached leaflets. Six cultivars previously demonstrated to represent range of resistance to Sclerotinia minor or S. sclerotiorum in the field or in the laboratory were used: highly resistant, Georgia 03L, ARSOK-R35; moderately resistant, Red River Runner, Tamrun 96; and susceptible, Tamrun OL02, and Okrun. Preliminary results indicate that the petiole inoculations, whether using whole plants or detached stems, were more consistent with previous field and laboratory results than the leaflet inoculations. Results will help determine the most consistent and efficient method for assaying physiological resistance to Sclerotinia blight in peanut.