Location: Sugarcane ResearchTitle: Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids
|ZHOU, H - Guangxi Academy Of Agricultural Sciences|
|CHEN, C - Auburn University|
|LU, X - Yunnan Academy Of Agriculture Sciences|
|CAI, Q - Yunnan Academy Of Agriculture Sciences|
|ZHU, J - Yunnan Academy Of Agriculture Sciences|
|CHEN, P - Fujian Agricultural & Forestry University|
|CHEN, R - Fujian Agricultural & Forestry University|
Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2014
Publication Date: 6/18/2014
Citation: Pan, Y.-B., Zhou, H., Chen, C.Y., Lu, X., Cai, Q., Zhu, J., Chen, P.H., Chen, R.K. 2014. Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids. Journal of the American Society of Sugar Cane Technologists. 34:64-65.
Technical Abstract: Although the microsatellite (SSR) DNA markers have been extensively used in sugarcane breeding research, little is known about how SSR markers are being transmitted into reproductive and zygotic cells. To illustrate this, a high-throughput molecular genotyping experiment was conducted on 964 individually collected single pollen grains and a 288-self progeny mapping population (S1) of a Louisiana sugarcane cultivar LCP 85-384. Through PCR, five pairs of polymorphic SSR primers, namely, SMC336BS, SMC1604SA, SCM7, UGSM10, and UGSM97, primed the amplification of 20 discrete and reproducible SSR alleles. Of these, three alleles were from SMC336 BS, four from SMC1604SA, four from SCM7, three from UGSM97, and six from UGSM10, respectively. Preliminary analysis showed that only 11 out of the 20 SSR alleles segregated in accordance with Mendelian segregation ratios expected of S1 or pollen populations of a 2n = 10 polyploid genome. Fewer SSR genotypes were observed for each SSR marker within the S1 population than those within the pollen population. Interestingly, the most frequent genotype within the S1 population always resembled the genotype of LCP 85-384. In addition, higher polymorphism information content (PIC) values were observed in the pollen population than the S1 population, probably due to a higher incidence of shared alleles between female and male gametes. These results provide guidance for sugarcane breeders in pairing parental genotypes during crossing, estimating optimum size of seedling population, and defining selection criteria.