Location: Hard Winter Wheat Genetics ResearchTitle: Quantitative trait loci for Fusarium head blight resistance in Huangcandou x 'Jagger' wheat) Author
Submitted to: Crop Science
Publication Type: Peer reviewed journal
Publication Acceptance Date: 4/30/2014
Publication Date: 5/7/2014
Citation: Cai, J., Bai, G. 2014. Quantitative trait loci for Fusarium head blight resistance in Huangcandou x 'Jagger' wheat. Crop Science. DOI:10.20135/cropsci2013.12.0835. Interpretive Summary: Fusarium head blight (FHB), also called wheat scab, is a devastating disease in wheat, and growing resistant cultivars is the most effective strategy to control the disease. Huangcandou (HCD) is a Chinese wheat landrace that shows a high level of scab resistance. To determine genes conditioning scab resistance in HCD, we analyzed a population of recombinant inbred lines (RILs) for scab resistance and using DNA markers. The population was developed from the cross between HCD and ‘Jagger’, a susceptible hard winter wheat from Kansas. Marker analysis identified three chromosome regions from HCD and two from ‘Jagger’ that were associated with scab resistance. In HCD, scab resistance was associated with two regions on the short arms of chromosomes 3B, and one on the short arm of chromosome 3A. These regions explained 10% to 26.1% of phenotypic variation for scab resistance. ‘Jagger’ also has two chromosome regions (chromosomes 2D and 6D) contributing to scab resistance that explained less than 10% of phenotypic variations. A combination of scab resistance genes from both HCD and ‘Jagger’ can enhance scab resistance in wheat.
Technical Abstract: Fusarium head blight (FHB) is a devastating disease in wheat (Triticum aestivum), and growing resistant cultivars is one of the most effective strategies to minimize its damage. Huangcandou (HCD) is a Chinese wheat landrace that shows a high level of resistance to FHB spread within a spike (type II). To identify quantitative trait loci (QTLs) for FHB resistance in HCD, 190 recombinant inbred lines (RILs) were developed from HCD x ‘Jagger’. ‘Jagger’ is a susceptible hard winter wheat (HWW) from Kansas. The population was evaluated for percentage of symptomatic spikelets (PSSs) per spike after single-floret inoculation in three greenhouse experiments. Initial marker screening identified 261 polymorphic simple-sequence repeats (SSRs) between parents. Analysis of these markers in the RIL population identified five QTLs, three from HCD and two from ‘Jagger’ chromosomes. Two of the three QTL from HCD were mapped on the short arms of chromosomes 3B, one in the distal end (3BSd) and another near centromere (3BSc); the third was on the short arm of 3A (3AS). The QTL on 3BSd coincides with a previously reported Fhb1 and explained 26.1% of phenotypic variation. The QTL on 3AS explained up to 10.0% of phenotypic variation. The two QTLs from ‘Jagger’ on chromosomes 2D and 6D explained 9.5% and 6.7% of phenotypic variations, respectively. A combination of QTLs from HCD and Jagger can enhance FHB type II resistance in wheat.