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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition, Growth and Physiology » Research » Publications at this Location » Publication #303289

Title: Effects of change in body composition on gene expression in the uterine endometrium of beef cattle

item BRIDGES, G - University Of Minnesota
item KRUSE, S - University Of Minnesota
item McNeel, Anthony
item Amundson, Olivia
item FUNNELL, B - University Of Minnesota
item BIRD, S - University Of Minnesota
item Cushman, Robert - Bob

Submitted to: International Symposium on Reproduction in Domestic Ruminants
Publication Type: Abstract Only
Publication Acceptance Date: 4/16/2014
Publication Date: 8/1/2014
Citation: Bridges, G.A., Kruse, S.G., McNeel, A.K., Amundson, O.L., Funnell, B.J., Bird, S.L., Cushman, R.A. 2014. Effects of change in body composition on gene expression in the uterine endometrium of beef cattle. In: Juengel, J. L., Miyamoto, A., Price, C., Reynolds, L. P., Smith, M. F., and Webb, R., editors. Reproduction in Domestic Ruminants VIII. Leicestershire, England: Context Products Ltd. p. 554.

Interpretive Summary:

Technical Abstract: The objective of this study was to determine the impact of change of body composition on gene expression in the uterine endometrium of beef cows. Mature, non-lactating Angus cows (body condition score [BCS] = 5.07 ± 0.1) were fed a similar diet for 30 d prior to the initiation of the study. Following the first endometrial biopsy (Col1; d 0), half of the cows (lose-gain; L-G; n = 12) were fed at 80% of NCR requirements while the other half (gain-lose; G-L; n = 12) were fed at 120% of NCR requirements for 87 d. From d 87 to 115, cows were fed to maintain current weight and BCS. From d 115 to 210 cows in the L-G treatment were fed at 120% and cows in the G-L treatment were fed 80% NCR requirements. From d 210 to 246, cows were fed to maintain current weight and BCS. On d 16 of the estrous cycle, cows were administered 50 mg of PGF2a, 48 h later given 100 µg of GnRH, and endometrial biopsy collection began 2 h after GnRH. During the experiment, biopsies were collected at three time points, Col1 (d 0), Col2 (d 115) and Col3 (d 246). Total cellular RNA was extracted from the endometrial biopsies and real-time RT-PCR was performed to determine mRNA abundance for oxytocin receptor (OXTR), fibroblast growth factor 10 (FGF10), prostaglandin-endoperoxide synthase 2 (PTGS2), insulin-like growth factor binding protein 3 (IGFBP3), and glyceraldehyde 3-phsophate dehydrogenase (GAPDH). Data was analyzed using the MIXED procedure of SAS with treatment, collection, and the interaction in the statistical model. Repeated measure analysis was used when appropriate. As designed, BCS was similar between L-G and G-L treatments at Col1and Col3, but differed (P < 0.05) at Col2 (4.2 ± 0.1; 6.1 ± 0.1, respectively). Body weight, rib fat, and rump fat followed a similar relationship. Estradiol concentrations at time of endometrial biopsy did not differ between treatments. Relative abundance of FGF10 mRNA was not impacted by collection, but was greater (P < 0.05) in G-L than L-G. There was a treatment by collection interaction (P < 0.05) resulting in greater relative abundance of PTGS2 mRNA at Col2 in the L-G than G-L treatment. There was an effect of collection (P < 0.05) on mRNA abundance of IGFBP3, and OXTR was not impacted by treatment or collection. Across treatments and collections, PTGS2 was negatively correlated (P < 0.05) with BCS, body weight, and estradiol concentrations. In conclusion, alterations in body composition resulted in changes to the endometrium transcriptome that may mediate the effects of nutrition on uterine function.