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ARS Home » Midwest Area » Lexington, Kentucky » Forage-animal Production Research » Research » Publications at this Location » Publication #303072

Research Project: Optimizing the Biology of the Animal-Plant Interface for Improved Sustainability of Forage-Based Animal Enterprises

Location: Forage-animal Production Research

Title: Effect of acute exposure to ergot alkaloids on short-chain fatty acid absorption and barrier function of isolated bovine ruminal epithelium

item FOOTE, ANDREW - University Of Kentucky
item PENNER, GREGORY - University Of Kentucky
item WALPOLE, M - University Of Kentucky
item Klotz, James
item BUSH, LOWELL - University Of Kentucky
item HARMON, DAVID - University Of Kentucky

Submitted to: Joint Meeting of the ADSA, AMSA, ASAS and PSA
Publication Type: Abstract Only
Publication Acceptance Date: 3/25/2014
Publication Date: 7/23/2014
Citation: Foote, A.P., Penner, G.P., Walpole, M.E., Klotz, J.L., Bush, L.P., Harmon, D.L. 2014. Effect of acute exposure to ergot alkaloids on short-chain fatty acid absorption and barrier function of isolated bovine ruminal epithelium. Joint Meeting of the ADSA, AMSA, ASAS and PSA. 92(E.Suppl.2):874.

Interpretive Summary:

Technical Abstract: Ergot alkaloids present in endophyte-infected tall fescue are the causative agents for fescue toxicosis in cattle. Ergot alkaloids have been shown to cause a reduction in blood flow to the rumen epithelium as well as a decrease in short-chain fatty acid (SCFA) absorption from the washed rumen of steers. It is possible that ergot alkaloids could negatively impact SCFA transport pathways and the barrier function of the rumen epithelium. An experiment was conducted to determine if acute exposure to an endophyte-infected tall fescue seed extract (EXT) would affect total, passive, or facilitated acetate and butyrate flux across the isolated bovine rumen epithelium as well as the barrier function measured by inulin flux and tissue conductance (Gt). Flux of ergovaline across the ruminal epithelium was also evaluated. Ruminal tissue from the caudal dorsal sac of Holstein steers (n = 6), fed a common diet, was collected and isolated shortly after slaughter and mounted between two halves of Ussing chambers under voltage clamp conditions. In vitro treatments included vehicle control (0.4% methanol), Low EXT (50 ng ergovaline/mL), and High EXT (250 ng ergovaline/mL). Results indicate that there was no effect of an acute exposure to ergot alkaloids on total, passive, or facilitated flux of acetate or butyrate across the isolate bovine rumen epithelium (P > 0.51). Inulin flux (P = 0.16) and Gt (P > 0.17) also were not affected by EXT treatment, indicating no alteration in barrier function due to acute ergot alkaloid exposure. Based on ergovaline concentrations measured in the serosal buffer of the High EXT treatment, the flux rate is approximately 0.25 – 0.44 ng/(cm2 • h). These data indicate that specific pathways for SCFA absorption and barrier function of the ruminal epithelium are not affected by acute exposure to an extract of tall fescue seed at the concentrations tested. Ergovaline has the potential to cross the rumen epithelium of cattle which could contribute to the reduced foregut blood flow seen in previous experiments and lead to reduced growth rates of cattle.