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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #302620

Research Project: Genetics and Management of Soybean Cyst Nematodes and Diseases for Sustainable Production

Location: Crop Genetics Research

Title: Overexpression of a soybean salicylic acid methlyltransferase gene confers resistance to soybean cyst nematode

Author
item Jingyu, Lin - University Of Tennessee
item Mazarei, Mitra - University Of Tennessee
item Zhao, Nan - University Of Tennessee
item Rudis, Mary - University Of Tennessee
item Pantalone, Vincent - University Of Tennessee
item Arelli, Prakash
item Chen, Feng - University Of Tennessee
item Stewart Jr., Charles - University Of Tennessee

Submitted to: Current Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2014
Publication Date: 6/4/2014
Citation: Jingyu, L., Mazarei, M., Zhao, N., Rudis, M., Pantalone, V., Arelli, P.R., Chen, F., Stewart Jr., C. 2014. Overexpression of a soybean salicylic acid methlyltransferase gene confers resistance to soybean cyst nematode. Current Biology. Page 10.

Interpretive Summary:

Technical Abstract: Soybean cyst nematode (Heterodera glycines Ichinohe, SCN) is the most pervasive pest of soybean [Glycine max (L.) Merr.] in the USA and worldwide. SCN reduced soybean yields worldwide by an estimated billion dollars annually. These losses remained stable with the use of resistant cultivars but over time genetically heterogeneous nematode populations will adapt to deployed resistance alleles. Traditional breeding methodologies combined with genetic engineering techniques should be adapted to providing durable resistance to SCN in soybean. A salicylic acid methyl transferase gene (GmSAMT1) was identified from soybean as a candidate soybean cyst nematode defense-related gene in our previous analysis using GeneChip microarray experiments. Using in vitro enzyme assay, the Escherichia coli expressed GmSAMT1 was confirmed to function as salicylic acid methyl transferase catalyzing the conversion of salicylic acid to methyl salicylate. The transgenic hairy roots overexpressing GmSAMT1 were tested for the soybean response to SCN race 2, race 3 and race 5 by analyzing the proportion of nematodes developed beyond J3 stage at 15 days post-inoculation. Different levels of increased resistance were found. Stably-transformed soybean were produced using Agrobacterium tumefaciens whereby GmSAMT1 was overexpressed and T2 plants were assayed against SCN race 2 and race 3, respectively. The elevated resistance was also detected through analyzing the nematode reproduction at 35 and 42 days post-inoculation. Taken together, these data suggest that the increase in expression of GmSAMT1 contributed to the soybean resistance against SCN.