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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Publications at this Location » Publication #302495

Title: Chapter 18: Culture and Propagation of Microsporidia

Author
item MOLESTINA, ROBERT - American Type Culture Collections
item Becnel, James
item WEISS, LOUIS - Albert Einstein College Of Medicine

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 5/1/2014
Publication Date: 9/1/2014
Citation: Molestina, R., Becnel, J.J., Weiss, L.M. 2014. Chapter 18: Culture and Propagation of Microsporidia. Book Chapter. 2:457-467.

Interpretive Summary: The science of microsporidiology encompasses a diverse assemblage of pathogens from a large and varied group of hosts. The cultivation of microsporidia in vitro has served a critical role in studying the life cycle, metabolism, and pathogenesis of these parasites. Culture isolation has served as an important addition to the diagnosis of infection in human microsporidiosis and to establishing repositories of isolates to be used for antigenic, molecular, biochemical, and epidemiological studies. The routine use of cell cultures for propagation of microsporidia of insect or mammalian origin did not occur until the 1950s and 1960s. Many members of this group have been studied and exploited for their role in the control of insect pests and vectors as well as their detrimental impact on vertebrates including man. This chapter provides an overview on cell culture of microsporidia in vertebrate and invertebrate cells.

Technical Abstract: The science of microsporidiology encompasses a diverse assemblage of pathogens from a large and varied group of hosts. The cultivation of microsporidia in vitro has served a critical role in studying the life cycle, metabolism, and pathogenesis of these parasites. Culture isolation has served as an important addition to the diagnosis of infection in human microsporidiosis and to establishing repositories of isolates to be used for antigenic, molecular, biochemical, and epidemiological studies. The routine use of cell cultures for propagation of microsporidia of insect or mammalian origin did not occur until the 1950s and 1960s. Many members of this group have been studied and exploited for their role in the control of insect pests and vectors as well as their detrimental impact on vertebrates including man. This chapter provides an overview on cell culture of microsporidia in vertebrate and invertebrate cells.