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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #302400
Title: Quantitative trait loci underlying seed sugars content in MD96-5722 by spencer recombinant inbred line population of soybean

Author
item AKOND, MASUM - Fayetteville State University
item KANTARTZI, STELLA - Southern Illinois University
item MEKSEM, KHALID - Southern Illinois University
item Bellaloui, Nacer
item LIGHTFOOT, DAVID - Southern Illinois University
item KASSEM, ABDELMAJID - Fayetteville State University

Submitted to: Food and Nutrition Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/8/2015
Publication Date: 8/13/2015
Citation: Akond, M., Kantartzi, S.K., Meksem, K., Bellaloui, N., Lightfoot, D.A., Kassem, A.M. 2015. Quantitative trait loci underlying seed sugars content in MD96-5722 by spencer recombinant inbred line population of soybean. Food and Nutrition Sciences. 964-973.

Interpretive Summary: Soybean seeds contain desirable and undesirable sugars. Sugars such as sucrose are desirable because they improve taste and flavor in tofu, soymilk, and natto. Other sugars such as raffinose and stachyose are undesirable because they reduce the nutritive value of the meal and are indigestible by human and animals, causing flatulence or diarrhea in non-ruminants. Increasing the level of desirable sugars in soybean without affecting other seed components remains a challenge. Therefore, identifying the genes controlling the levels of these sugars in seeds is critical. The objective of this research was to identify regions of the genes (quantitative trait loci, QTL) controlling sucrose, raffinose, and stachyose in soybean breeding populations using DNA markers (Single Nucleotide Polymorphism, SNP). The results showed that three regions (linkage groups or QTL) for seed sucrose content were identified on separate chromosomes, seven regions were identified for raffinose, and four regions were identified for stachyose. This information can facilitate breeding soybean lines with high levels of sucrose and lower levels of raffinose and stachyose.

Technical Abstract: Sucrose, raffinose, and stachyose are important soluble sugars in soybean [Glycine max (L.) Merr.] seeds, and soybean seeds with higher sucrose and lower raffinose and stachyose are desirable. Therefore, optimizing sugars biosynthesis is a major goal for soy food industry. The objective of this study was to identify genomic regions containing quantitative trait loci (QTL) controlling sucrose, raffinose, and stachyose in a set of F5:7 recombinant inbred lines (RILs) derived from a cross between MD 96-5722 and ‘Spencer’ using 5,376 Single Nucleotide Polymorphism (SNP) markers from the Illumina Infinium SoySNP6K BeadChip array. The MD 96-5722 by ‘Spencer’ high-density SNP-based genetic map is composed of 16 Linkage groups (LG) on which fourteen significant QTL were identified and mapped in eight different LGs or chromosomes (Chr). Three QTL for seed sucrose content were identified on LGs N (Chr3), K (Chr9) and E (Chr15). Seven QTL was identified for raffinose on LGs D1a (Chr1), N (Chr3), C2 (Chr6), K (Chr9), B2 (Chr14) and J (Chr16). Four QTL for Stachyose were identified on LG D1a (Chr1), C2 (Chr6), H (Chr12), and B2 (Chr14). Information regarding significant QTL could facilitate breeding strategies to develop soybean lines with high levels of sucrose and lower levels of indigestible sugars raffinose and stachyose.