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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #302050

Research Project: Understanding Genetic and Physiological Factors Affecting Nutrient Use Efficiency of Dairy Cattle

Location: Animal Genomics and Improvement Laboratory

Title: Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene

Author
item ZHANG, LIANGZHI - Northwest Agricultural & Forestry University
item YANG, MINGJUAN - Northwest Agricultural & Forestry University
item Li, Congjun - Cj
item XU, YAO - Northwest Agricultural & Forestry University
item JIA, SHANGANG - Northwest Agricultural & Forestry University
item SUN, JIAJIE - Northwest Agricultural & Forestry University
item LAN, XIANYONG - Northwest Agricultural & Forestry University
item LEI, CHUZHAO - Northwest Agricultural & Forestry University
item CHEN, HONG - Northwest Agricultural & Forestry University

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/6/2013
Publication Date: 12/16/2013
Citation: Zhang, L., Yang, M., Li, C., Xu, Y., Jia, S., Sun, J., Lan, X., Lei, C., Chen, H. 2013. Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene. Animal Genetics. 45:171-179. DOI.10.1111/age.12112.

Interpretive Summary: ADIPOQ is a very important gene in cattle. This gene activity affects beef quality. This study demonstrated that a region of the genome has variable duplication. This duplication can decrease ADIPOQ gene activity. Association studies also confirm this duplication affects cattle growth and body measurements. Overall, his study provides valuable genetic information to explore the growth regulation of cattle.

Technical Abstract: The ADIPOQ gene of cattle, is located in the vicinity of the quantitative trait locus (QTL) wich effects marbling, the rib eye muscle area and fat thickness on BTA1. In our study, a novel variable duplication (NW_003103812.1:g.9232067_9232133 dup) in the bovine ADIPOQ promoter region was identified and genotyped in seven Chinese cattle breeds. Using a reporter assay, we demonstrated that g.9232067_9232133 dup decreased the basal transcriptional activity of the ADIPOQ gene in 3T3-L1 and C2C12 cells. Furthermore, g.9232067_9232133 dup suppressed the mRNA expression of the gene in adipose and muscle tissues. Association analysis indicated that the g.9232067_9232133 dup were associated with body measurements (P < 0.05).