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Title: Physical mapping of peroxidase (POD) genes and development of functional markers for TaPod-D1 on bread wheat chromosome 7D

Author
item GENG, HONGWEI - Washington State University
item SHI, JIA - Xinjiang Agricultural University
item FUERST, E - Washington State University
item WEI, JINGXIN - Chinese Academy Of Agricultural Sciences
item Morris, Craig

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/4/2019
Publication Date: 4/24/2019
Publication URL: http://handle.nal.usda.gov/10113/6471169
Citation: Geng, H., Shi, J., Fuerst, E.P., Wei, J., Morris, C.F. 2019. Physical mapping of peroxidase (POD) genes and development of functional markers for TaPod-D1 on bread wheat chromosome 7D. Theoretical and Applied Genetics. 10:523. https://doi.org/10.3389/fpls.2019.00523.
DOI: https://doi.org/10.3389/fpls.2019.00523

Interpretive Summary: Arabinoxylans are an important group of non-starch polysaccharides present in wheat grain, and have the capacity to significantly affect the properties of dough and the final baked product. Arabinoxylan feruloyl transferase (AFT) in grain is an important enzyme in determining the structure of arabinoxylans, and is part of the BAHD family of acyltransferases. In this study, the full-length genomic DNA sequence of TaBAHD-A1 and TaBAHD-B1 on chromosome 3AL and 3BL were characterized by in silico cloning and experimental validation. As a result, TaBAHD-A1 and TaBAHD-B1 were found to be physically located on 0.21 0.26 and 0.07-0.22 fraction arm length of chromosomes 3AL and 3BL, respectively, based on PCR amplification of a set of group 3 nullisomic-tetrasomic lines, six group 3 ditelosomic lines, and 16 group 3 deletion lines of cv. Chinese Spring. This study provides useful information about the molecular mechanism of BAHD genes, and will contribute a valuable resource for wheat quality improvement and breeding.

Technical Abstract: Arabinoxylans are an important group of non-starch polysaccharides present in wheat grain, and have the capacity to significantly affect the properties of dough and the final baked product. Arabinoxylan feruloyl transferase (AFT) in grain is an important enzyme in determining the structure of arabinoxylans, and is part of the BAHD family of acyltransferases. In this study, we report the molecular characterization and physical mapping of BAHD genes in common wheat (Triticum aestivum L.). The full length genomic DNA (gDNA) sequence of two BAHD genes, one each located on chromosomes 3A and 3B, designated TaBAHD-A1 and TaBAHD-B1, respectively, were characterized by in silico comparisons to rice (Oryza sativa L.) and experimental validation. The cloned TaBAHD A1 and TaBAHD-B1 genes comprise 1,429 bp and 1,432 bp, respectively. Both of them possess an open reading frame (ORF) of 1,266 bp, including 2 exons and 1 intron. The deduced polypeptides contain 422 amino acid residues with a predicted molecular weight of ~45.1 kDa and a HXXXD domain (His159 to Asp163). TaBAHD-A1 and TaBAHD-B1 were found to be physically located on 0.21 0.26 and 0.07-0.22 fraction arm length of chromosomes 3AL and 3BL, respectively, based on PCR amplification of a set of group 3 nullisomic-tetrasomic lines, six group 3 ditelosomic lines, and 16 group 3 deletion lines of cv. Chinese Spring. This study provides useful information about the molecular mechanism of BAHD genes, and will contribute a valuable resource for wheat quality improvement and breeding.