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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #301464

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources Laboratory

Title: Molecular detection and characterization of Chinese Yam mild mosaic virus isolates

Author
item Li, Ruhui
item WANG, MINGQIANG - South China Agricultural University
item LI, FAN - Yunnan Agricultural University
item ZHOU, GUOHUI - South China Agricultural University
item LAN, PINGXIU - Yunnan Agricultural University
item XU, DONGLIN - South China Agricultural University

Submitted to: Virus Genes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/27/2014
Publication Date: 9/23/2014
Publication URL: http://onlinelibrary.wiley.com/doi/10.1111/jph.12337/epdf
Citation: Li, R., Wang, M., Li, F., Zhou, G., Lan, P., Xu, D. 2014. Molecular detection and characterization of Chinese Yam mild mosaic virus isolates. Virus Genes. DOI: 10.1111/jph.12337.

Interpretive Summary: Yams (Dioscorea spp.) are a group of multi-species tuber crops in many tropical and sub-tropical countries. Some yams are important medical plants. The crops are propagated by tubers, and their production is strongly limited by virus accumulation. In this study, a RT-PCR was developed to detect Yam mild mosaic virus (YMMV). Genetic information of 19 Chinese isolates of YMMV from different areas was obtained, and analysis showed that these isolates were different from those from America and Pacific Islands. These isolates were also different from each other, and formed two distinct groups. The complete genomic sequences of two representative isolates of each of the two groups were determined. Analyses of the genomic information confirmed that they were distinct. The study provides the information necessary for evolution study of the virus. The information is also used to develop a better detection and diagnostic method for the virus.

Technical Abstract: An improved RT-PCR was developed and validated to be sensitive and reliable for the detection of Yam mild mosaic virus (YMMV). Sequences of coat protein core region of 19 Chinese isolates were obtained, and analysis indicated the presence of different genetic variants. Phylogenetic analyses showed that the Chinese isolates were divided into two distinct clusters. Complete genomic sequences of two Chinese variants were determined to be 9526 and 9529 nucleotides long, excluding the 3’ poly (A) tail. Their genomic structure and organization were virtually identical to that of a Brazilian isolate. The two variants shared identity of 87.3% to one another and 83.9-84.6% to the Brazilian variant at the genomic sequence level. Phylogenetic analyses supported that they represented two distinct YMMV lineages.