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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #300392

Research Project: Development and Characterization of Soybean Germplasm, Curation of Stored Accessions, and Regional Evaluations of New Genotypes

Location: Crop Genetics Research

Title: Draft genome sequence of Phomopsis longicolla MSPL 10-6

Author
item Li, Shuxian
item Darwish, Omar - Towson University
item Alkharouf, Nadim - Towson University
item Matthews, Benjamin - Ben
item Ji, Pingsheng - University Of Georgia
item Domier, Leslie
item Zhang, Ning - Rutgers University
item Bluhm, Burton - University Of Arkansas

Submitted to: Genomics Data
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/10/2014
Publication Date: 3/1/2015
Citation: Li, S., Darwish, O., Alkharouf, N., Matthews, B.F., Ji, P., Domier, L.L., Zhang, N., Bluhm, B.H. 2015. Draft genome sequence of Phomopsis longicolla MSPL 10-6. Genomics Data. 3:55-56.

Interpretive Summary: Phomopsis seed decay of soybean is a major cause of poor quality of soybean seeds. The disease is caused primarily by a fungus (mold), but information about how the disease develops and how the fungus causes the disease are lacking. In the study, we used a molecular approach to sequence the fungus from Mississippi. The genetic make-up of the fungus was investigated and analyzed. We identified genes that are associated with plant cell wall degradation and discovered new genes that may play an important role in the cause of the disease. Information obtained from this study is valuable for understanding the genetic basis of the fungus causing poor seed quality, and development of new control strategies for this pathogen.

Technical Abstract: Phomopsis longicolla T.W. Hobbs is the primary cause of Phomopsis seed decay in soybean. We report the de novo assembled draft genome sequence of P. longicolla isolate MSPL10-6 with a 54.8-fold depth of coverage. The resulting draft genome was estimated to be approximately 64 Mb in size with an overall G+C content of 47.9%. The draft genome contains 18,065 annotated genes, of which 271 genes are associated with plant cell wall degradation, and 3,407 are novel sequences that did not match any reported genes in available databases. The genome sequences of P. longicolla is valuable for molecular genetic analysis, marker development, manipulation of pathogenicity-related genes and development of new control strategies for this pathogen.