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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #300293

Title: Blueberry mosaic associated virus – A putative, new member of Ophioviridae

Author
item THEKKE VEETIL, THANUJA - University Of Arkansas
item Polashock, James
item Keller, Karen
item Martin, Robert
item HO, THIEN - University Of Arkansas
item TZANETAKIS, IOANNIS - University Of Arkansas

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2014
Publication Date: 5/25/2016
Citation: Thekke Veetil, T., Polashock, J.J., Keller, K.E., Martin, R.R., Ho, T., Tzanetakis, I.E. 2016. Blueberry mosaic associated virus – A putative, new member of Ophioviridae. Acta Horticulturae. 1117:103-110. doi: 10.17660/ActaHortic.2016.1117.18.

Interpretive Summary: Blueberry mosaic initially was reported more than 50 years ago and is now known from different parts of the world. A new virus, closely associated with the disease has been identified recently. The virus tentatively named as Blueberry mosaic associated virus (BlMaV), is a putative member of the genus Ophiovirus with a genome comprising of three negative-sense RNAs (RNAs 1-3). Genome organization and analysis indicates that BlMaV resembles Citrus psorosis virus, the type member of the family Ophioviridae. RNA 1 encodes two proteins, a 23 kDa protein of unknown function and a 272 kDa RNA-dependent RNA polymerase. RNAs 2 and 3 encode for a putative 58 kDa protein of unknown function and the 50 kDa coat protein, respectively. Phylogenetic analyses conducted on 1200 nucleotides in coding regions of RNAs 2 and 3 of 59 U.S. and Slovenian isolates revealed significant amino acid conservation (96-100% and 95-100% identity, respectively) among isolates.

Technical Abstract: Blueberry mosaic initially was reported more than 50 years ago and is now known from different parts of the world. A new virus, closely associated with the disease has been identified recently. The virus tentatively named as Blueberry mosaic associated virus (BlMaV), is a putative member of the genus Ophiovirus with a genome comprising of three negative-sense RNAs (RNAs 1-3). Genome organization and analysis indicates that BlMaV resembles Citrus psorosis virus, the type member of the family Ophioviridae. RNA 1 encodes two proteins, a 23 kDa protein of unknown function and a 272 kDa RNA-dependent RNA polymerase. RNAs 2 and 3 encode for a putative 58 kDa protein of unknown function and the 50 kDa coat protein, respectively. Phylogenetic analyses conducted on 1200 nucleotides in coding regions of RNAs 2 and 3 of 59 U.S. and Slovenian isolates revealed significant amino acid conservation (96-100% and 95-100% identity, respectively) among isolates.