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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Meat Safety & Quality Research » Research » Publications at this Location » Publication #300287

Title: Escherichia coli O157:H7, diet, and fecal microbiome in beef cattle

item Wells, James - Jim
item Kim, Min
item Bono, James - Jim
item Kuehn, Larry
item BENSON, ANDY - University Of Nebraska

Submitted to: Joint Meeting of the ADSA, AMSA, ASAS and PSA
Publication Type: Proceedings
Publication Acceptance Date: 12/23/2013
Publication Date: 3/1/2014
Citation: Wells, J., Kim, M.S., Bono, J.L., Kuehn, L.A., Benson, A.K. 2014. Escherichia coli O157:H7, diet, and fecal microbiome in beef cattle. In: Proceedings of American Society of Animal Science, Meat Science and Muscle Biology Symposium. Journal of Animal Science. 92:1345-1355.

Interpretive Summary: Not applicable

Technical Abstract: Shiga-toxigenic Escherichia coli, such as E. coli O157:H7, are foodborne zoonotic pathogens that can cause severe illness and death in humans. The gastrointestinal tract of ruminant animals has been identified as a primary habitat for E. coli O157:H7, and in cattle the terminal gastrointestinal tract appears to be a primary site for colonization. This pathogen has been found in cattle feces, on cattle hides, and in the production environment, and transmission to humans has occurred as a result of consumption of contaminated ground beef, water, and produce. Interventions to reduce the pathogen at beef harvest have significantly reduced the occurrence of the pathogen, but outbreaks and recalls due to the pathogen still occur for beef products. Interventions prior to harvest in the feedyard have had little success, but critical control points for implementing interventions are limited compared to the beef plant. The percentage of animals shedding E. coli O157:H7 in the feces can be highly variable from pen to pen, and the levels in the feces can vary from animal to animal. Animals colonized and shedding E. coli O157:H7 at high levels are a small fraction of animals in a pen, but are important source for transferring the pathogen amongst the penmates. Recent research has indicated that diet may greatly influence the shedding of E. coli O157:H7. In addition, diet can influence the microflora composition in the feces. However, little is known about the interaction between the indigenous microflora and fecal shedding of E. coli O157:H7. Understanding the influence of indigenous microflora on the colonization and shedding of E. coli O157:H7 will provide a potential avenue for intervention in the preharvest production environment not yet exploited.