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United States Department of Agriculture

Agricultural Research Service

Research Project: Enhancing Plant Resistance to Water-Deficit and Thermal Stresses in Economically Important Crops

Location: Plant Stress and Germplasm Development Research

Title: A major locus for fusarium wilt race 4 resistance identified in Gossypium Hirsutum Acala NemX using an interspecific progeny with g barbadense)

item Wang, Congli
item Ulloa, Mauricio
item Roberts, Philip

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/8/2014
Publication Date: 5/15/2014
Citation: Wang, C., Ulloa, M., Roberts, P.A. 2014. A major locus for fusarium wilt race 4 resistance identified in Gossypium Hirsutum Acala NemX using an interspecific progeny with G barbadense [abstract]. National Cotton Council Beltwide Cotton Conference, January 6-8, 2014, New Orleans, Lousiana. No. 15614.

Interpretive Summary:

Technical Abstract: Fusarium wilt, caused by the soil-borne pathogen Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4), is a vascular disease of cotton (Gossypium spp.) which causes plant injury and yield loss in most Pima (G. barbadense L.) and Acala or Upland (G. hirsutum L.) cultivars without co-infection with root-knot nematode (Meloidogyne incognita). QTL analysis on an interspecific progeny from a cross of Pima S7 × Acala NemX revealed a major locus conferring resistance to FOV4 in Acala NemX. Two SSR markers (BNL2650_196 and CIR112_226) were closely linked to the resistance locus, which accounted for 18 percent (LOD greater 3.5) to 40 percent (LOD greater 9.70) of resistance response of FOV4 foliar disease index at 10 and 20 days after inoculation (DAI), and up to 35 percent for the ratio of vascular discoloration/plant height (VDL/PH) using interval mapping analysis. Additional minor QTLs contributing to the resistance were identified at early DAI (10 and 13) on chromosomes 8, 11, and 21. Positive alleles of these minor QTLs were contributed by either Pima S-7 or Acala NemX. Genetic linkage analysis based on this progeny suggests that the major resistance locus may be located on chromosome 17. BNL2650 and CIR112 alleles were linked to previously reported SSRs CIR180 and BNL0834 (BNL0834 also was linked to resistance locus Fov4 in Pima S6). The BNL2650 and CIR112 SSR markers provide additional valuable resources for incorporating FOV4 resistance into elite germplasm using marker-assisted selection. In addition, some recombinant inbred lines from the Pima S7 x Acala NemX population with resistance to both root-knot nematode and Fusarium wilt races 1 and 4 provide multiple resistance sources for breeding.

Last Modified: 05/27/2017
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