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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #300013

Research Project: Improving Immunity, Health, and Well-Being in Cattle and Swine

Location: Livestock Issues Research

Title: Yeast cell wall supplementation alters immune parameters in response to a salmonella challenge in weaned pigs

Author
item Sanchez, Nicole
item Carroll, Jeffery - Jeff Carroll
item Corley, Jimmie - Lesaffre Yeast
item Broadway, Paul - Texas Tech University
item Butyn, Joe - University Of Nebraska
item Schmidt, Ty - University Of Nebraska
item Callaway, Todd

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 11/27/2013
Publication Date: 7/1/2014
Citation: Sanchez, N.C., Carroll, J.A., Corley, J.R., Broadway, P.R., Butyn, J.O., Schmidt, T.B., Callaway, T.R. 2014. Yeast cell wall supplementation alters immune parameters in response to a salmonella challenge in weaned pigs. Journal of Animal Science Supplement. 92(E-Suppl. 1):27-28 (Abstract #74).

Interpretive Summary:

Technical Abstract: The objective of this experiment was to evaluate the immune response of pigs supplemented with yeast cell wall (YCW) when challenged with Salmonella. Weaned pigs (n=39; 7.1+/-0.1 kilograms body weight) were individually housed in pens (1.2x0.6 meters) in an environmentally-controlled facility equipped with nipple waterers and feeders allowing ad libitum water and feed intake. Pigs were weighed upon arrival, blocked by body weight, and assigned to one of three treatments (n = 12/treatment): Control diet, which was a non-medicated starter diet (Control); control diet supplemented with YCW at 250 miligrams/kilogram (YCW250); and control diet supplemented with YCW at 500 milograms/kilogram (YCW500). Pigs were fed for 18 days, and pigs and feeders were weighed weekly. Pigs were anesthetized on day 7 to insert intraperitoneal temperature recording devices (TEMP) and on day 14 to insert jugular cannulas. On day 15 blood samples were collected at 6 hour intervals from -6 to 72 hours relative to oral Salmonella typhimurium (10^6 colony forming unites/pig) challenge. Serum was analyzed for cortisol, and whole blood was utilized to measure complete blood cell counts. Data were analyzed using the Mixed procedure of SAS specific for repeated measures. Control pigs had greater (P=0.006) average daily gain prior to the challenge than YCW500 pigs. Baseline TEMP was greater (P<0.001) in YCW250 pigs than Control and YCW500 pigs. There was a treatment x time interaction for the change in TEMP (P<0.01) and post-challenge cortisol (P=0.03), white blood cells (WBC; P=0.03), and neutrophils (P=0.02). Control pigs had greater (P=0.048) cortisol at 0 hour than both YCW-supplemented groups but had less (P=0.039) cortisol compared to YCW500 pigs at 24 and 30 hours post-challenge. Control pigs had greater (P=0.020) WBC counts than both YCW-supplemented groups 6 and 12 hours post-challenge, and YCW250 pigs had lower (P=0.011) WBC counts than Control and YCW500 pigs 18 hours post-challenge. Neutrophil counts were greater (P=0.043) in Control pigs than both YCW-supplemented groups at 6 and 12 hours post-challenge and greater (P=0.022) than YCW250 pigs at 18 hours post-challenge. Lymphocytes were greater (P<0.001) in Control and YCW500 pre- and post-challenge compared to YCW250 pigs. Control pigs had the greatest (P<0.001) monocyte counts compared to YCW-supplemented pigs. This study suggests that YCW supplementation decreases TEMP and specific WBC subtypes to an oral Salmonella challenge, which is similar to pervious results observed in cattle.