|AL RWAHNIH, MAHER - University Of California
|ROWHANI, ADIB - University Of California
|GOLINO, DEBORAH - University Of California
|ISLAS, CHRISTINA - University Of California
Submitted to: Canadian Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/13/2015
Publication Date: 1/21/2015
Publication URL: http://www.tandfonline.com/doi/full/10.1080/07060661.2014.999705#abstract
Citation: Al Rwahnih, M., Rowhani, A., Golino, D., Islas, C., Preece, J.E., Sudarshana, M.R. 2015. Detection and genetic diversity of Grapevine red blotch-associated virus isolates in table grape accessions in the National Clonal Germplasm Repository in California. Canadian Journal of Plant Pathology. 37:130-135.
Technical Abstract: The National Germplasm Repository of grapevines and Mediterranean tree fruits, and nut crops maintained by the U.S. Department of Agriculture at the Wolfskill ranch in Winters, California, holds the most genetically diverse collection of grapevines (Vitis spp., Family Vitaceae) in the World. Many of the accessions in the collection were obtained from historic collections of long standing on campus of the University of California at Davis as well as other sources that were created long before grapevine viruses were recognized and modern detection methods were developed. We examined 158 accessions of V. vinifera accessions that included 77 accessions that exhibited leafroll type symptoms and the rest randomly selected during dormant season for infection by Grapevine red blotch-associated virus (GRBaV), a recently discovered ssDNA virus of importance to wine grape production in California. PCR tests indicated 75 accessions were infected with GRBaV which included 33 accessions that had leafroll type symptoms. GRBaV-infected accessions included raisin, table and wine grape varieties and those colored white and black to red. A 557 bp amplicon, obtained from PCR assays, was purified and sequenced, and the genetic relationship of the GRBaV isolates was examined by constructing a phylogenetic tree based on neighbor joining method. The genetic variability among the isolates was only about 8%, which was not large, and the isolates belonged to five major clades. Although it is not yet known if GRBaV is present outside of North America, many accessions from international sources were positive for the virus.