Skip to main content
ARS Home » Research » Publications at this Location » Publication #299582
Title: Rdl-containing Fragment of GABA(A) from the Horn Fly, Haematobia Irritans, Susceptible Genotype

Author
item DOMINGUES, L - Louisiana State University Agcenter
item Guerrero, Felicito
item FOIL, LANE - Louisiana State University Agcenter

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 12/17/2012
Publication Date: 6/17/2013
Citation: Domingues, L.N., Guerrero, F., Foil, L.D. 2013. Rdl-containing Fragment of GABA(A) from the Horn Fly, Haematobia Irritans, Susceptible Genotype. National Center for Biotechnology Information (NCBI). http://www.ncbi.nlm.nih.gov. Accession Number KC346253.

Interpretive Summary: The horn fly, Haematobia irritans (L.), is a significant economic pest of cattle found throughout Europe, North Africa, Asia Minor, and the Americas. The major means of controlling the horn fly is through insecticides. A cyclodiene-containing ear tag product has been recently introduced and we are developing DNA-based methods to detect target site resistance to cyclodienes. Cyclodiene insecticides inhibit chloride ion transport by targeting a receptor known as the gamma-aminobutyric acid subtype A (GABAA) receptor. Target site resistance to cyclodienes (termed Rdl, resistance to dieldrin) in several species of insects is associated with a single amino acid substitution in a GABAA receptor locus. We have cloned and sequenced a 215 bp fragment from the horn fly GABAA receptor that encompasses the location of the Rdl gene mutation in cyclodiene resistant insects.

Technical Abstract: The horn fly, Haematobia irritans (L.), is a significant economic pest of cattle found throughout Europe, North Africa, Asia Minor, and the Americas. The major means of controlling the horn fly is through applications of chemicals with insecticidal activity. A cyclodiene-containing ear tag product has been recently introduced. Cyclodiene insecticides inhibit chloride ion transport regulated by the gamma-aminobutyric acid subtype A (GABAA) receptor and resistance to cyclodienes (termed Rdl) is associated with a single amino acid substitution in a GABAA receptor locus. We have cloned and sequenced a 215 bp fragment from the horn fly GABAA that encompasses the location of the Rdl gene mutation in cyclodiene resistant insects.