|LEE, SEONGHEE - University Of Arkansas|
|SING, PRATIBHA - Department Of Biotechnology - Spain|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/2013
Publication Date: 7/1/2013
Citation: Lee, S., Sing, P., Jia, M.H., Jia, Y. 2013. Analysis of rice PDR-like ABC transporter genes in sheath blight resistance. American Phytopathological Society Abstracts. 50-O.
Technical Abstract: Sheath blight caused by Rhizoctonia solani is one of the most damaging diseases of rice worldwide. To understand the molecular mechanism of resistance, we identified 450 differentially expressed genes in a resistant rice cultivar Jasmine 85 after R. solani infection with a combination of DNA microarray, serial analysis of gene expression, and suppression subtractive hybridization. Among the up-regulated rice genes, we found that transcripts of pleiotropic drug resistance (PDR)-like ATP-binding cassette (ABC) transporter rapidly accumulated at the early phases of R. solani infection throughout repeated time point experiments by semi-quantitative RT-PCR, and real time PCR. In contrast, alteration of transcripts of ABC transporters was not observed after Jasmine 85 was inoculated with both avirulent and virulent races of blast fungus at the same time points. One of the ABC transporters was also mapped to the major sheath blight resistant QTL qShB9-2 using a mapping population derived from the cross of a susceptible cultivar, Lemont with Jasmine 85. DNA sequences of ABC transporters from Jasmine 85 and Lemont were determined, and used to develop gene specific markers. The gene specific markers are being used to establish a correlation between disease reactions of 568 RILs of Jasmine85 and Lemont population and to determine the percentages of contribution of each member of these ABC transporter genes to sheath blight resistance.