Submitted to: Canadian Journal of Plant Science
Publication Type: Peer reviewed journal
Publication Acceptance Date: 6/17/2014
Publication Date: 6/25/2014
Citation: Jain, S., Porter, L., Kumar, A., Mir, R., Eigenbrode, S., Mcphee, K. 2014. Molecular characterization of genetic variation related to pea enation mosaic virus resistance in lentil (Lens culinaris Medik). Canadian Journal of Plant Science. DOI: 10.4141/CJPS-2014-077. Interpretive Summary: Lentil yield and quality are often adversely affected by Pea Enation Mosaic Virus (PEMV) due to premature plant death and reduced seed size, especially if co-infected with another virus such as bean leaf roll virus. Insecticide applications may be an effective means to reduce aphid colonization and population build up, but this approach is not cost effective or environmentally friendly. Adoption of PEMV-resistant cultivars can generate significant economic benefits and reduce production risks for growers. However, limited information regarding PEMV-resistant lentil lines has hindered identifying resistance gene(s) in the lentil genome and the subsequent transfer of resistance into commercial high-yielding varieties. The present research identified two lentil lines from Iran with resistance to PEMV under natural field conditions and several lentil lines from Iran and Chile with some levels of resistance under greenhouse screening conditions. The genetic diversity of these PEMV-resistant lines was determined and it was found that lentil germplasm from Iran, Chile, Ehiopia, India, Pakistan, Tukey, Afghanistan and Lebanon have a similar genetic make-up and germplasm from these countries is more likely to have genetic resistance to PEMV than germplasm from North America and Europe. Current commerical cultivars or advanced breeding lines can be breed with the presently discovered PEMV-resistant lines in lentil improvement programs to reduce lentil production risks due to this serious virus.
Technical Abstract: Identification of genetically diverse lentil germplasm with resistance to pea enation mosaic virus (PEMV) through combined approach of molecular marker analysis and phenotyping could prove useful in breeding programs. A total of 44 lentil (Lens culinaris Medik.) accessions, were screened for resistance to PEMV. Two accessions (PI 431663 and PI 432028) were identified with resistance to PEMV in field tests while several accessions were found with some level of resistance in greenhouse screenings. Thirty-six polymorphic SSR markers produced 43 loci with 2 to 12 alleles per locus. The polymorphic information content (PIC) values for these markers ranged from 0.22-0.85 with a mean of 0.55 per marker. Using allelic data of 36 SSR primer pairs, dissimilarity ranging from 0.12 to 0.74 was calculated. Cluster analysis performed using the unweighted pair group arithmetic mean method (UPGMA) divided the PEMV resistant accessions were grouped in one cluster along with other accessions from Iran, Chile, Ethiopia, India, Pakistan, Turkey, Afghanistan and Lebanon. All the adapted cultivars originating from North and South America were grouped in another cluster along with some European accessions. The 44 accessions were classified into 4 subpopulations using Structure 2.2 software complimenting the results of UPGMA analysis and indicated the effect of geographical origin on the grouping of accessions. The results of this study can be used to select genetically diverse PEMV resistant accessions for lentil improvement programs.