|WANG, ZHONGHUA - Zhejiang Wanli University|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 6/30/2013
Publication Date: 1/1/2014
Citation: Wang, Z., Jia, Y. 2014. Development and characterization of rice mutants for functional genomic studies and breeding. In: Tomlekova, N.B., Kozgar, M.I., Wani, M.R., editors. Mutagenesis: Exploring Novel Genes and Pathways. Wageningen Academic Publishers. p. 307-322.
Interpretive Summary: Many useful crop germplasm accessions have been lost during domestication and cultivation under changing climate. Creating genetically diverse plant materials is the basis for plant breeding worldwide. This study describes methods for creating and selecting agronomically important mutants using a chemical agent and irradiation. Resulting knowledge has benefited rice breeding and functional genomics studies including gaining a better understanding of molecular basis of rice blast disease resistance.
Technical Abstract: Mutagenesis is a powerful tool for creating genetic materials for studying functional genomics, breeding, and understanding the molecular basis of disease resistance. Approximately 100,000 putative mutants of rice (Oryza sativa L.) have been generated with mutagens. Numerous mutant genes involved in plant architecture, grain quality, and disease resistance have been isolated and characterized. Numerous rice cultivars with high yields and superior grain quality have been developed and effectively deployed for rice production worldwide. In this chapter, we describe 1) detailed methods using ethyl methanesulfonate (EMS), irradiation and fast neutrons for creating useful rice mutants; 2) methods for analysis of rice genes that are responsible for mutations; 3) utilization of these new mutants for rice breeding and functional genomics; and 4) molecular mechanisms of blast disease resistance gene-mediated defence responses.