Location: Plant Gene Expression CenterTitle: The C-terminal hypervariable domain targets Aradopsis ROP9 to the invaginated pollen tube plasma membrane Author
Submitted to: Molecular Plant
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/14/2013
Publication Date: 7/1/2013
Citation: Li, S., Zhou, L., Feng, Q., Mccormick, S.M., Zhang, Y. 2013. The C-terminal hypervariable domain targets Aradopsis ROP9 to the invaginated pollen tube plasma membrane. Molecular Plant. 6:1362-1364. Interpretive Summary: In pollen, a protein called Rop9, a class II Rop, is localized differently than is a type I Rop. Various gene constructs were studied to determine which part of the Rop9 protein is important for its localization.
Technical Abstract: Rop9 is a small GTPase of the Type II class, whereas the often studied type I Rops play roles during pollen tube growth. In pollen, Rop9 is located at the invaginated plasma membrane that surrounds the sperm cells, whereas type I Rops are located at the apical membrane of the pollen tube. The C-terminal end of Rop9 is responsible for its localization around the sperm cells, and this localization was independent of proteins called RhoGDIs and independent of its bound nucleotide.