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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #296845

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Identification and mapping of adult plant stripe rust resistance in soft red winter wheat cultivar ‘USG 3555’

Author
item CHRISTOPHER, M - Virginia Polytechnic Institution & State University
item LIU, S - Texas Agrilife Research
item HALL, M - Virginia Polytechnic Institution & State University
item Marshall, David
item Fountain, Myron
item JOHNSON, J - University Of Georgia
item MILUS, E - University Of Arkansas
item Garland-Campbell, Kimberly
item Chen, Xianming
item GRIFFEY, C - Virginia Polytechnic Institution & State University

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/16/2012
Publication Date: 2/1/2013
Citation: Christopher, M.D., Liu, S.Y., Hall, M.D., Marshall, D.S., Fountain, M.O., Johnson, J.W., Milus, E.A., Garland Campbell, K.A., Chen, X., Griffey, C.A. 2013. Identification and mapping of adult plant stripe rust resistance in soft red winter wheat cultivar ‘USG 3555’. Plant Breeding. 132:53-60.

Interpretive Summary: Little is known about the extent or diversity of resistance in soft red winter (SRW) wheat to stripe rust. The SRW wheat cultivar ‘USG 3555’ has effective adult-plant resistance to stripe rust, which was characterized in a population derived from ‘USG 3555’/‘Neuse’. The mapping population consisted of 99 recombinant inbred lines, which were evaluated for stripe rust infection type (IT) and severity to race PST-100 in field trials in North Carolina in 2010 and 2011. Genome-wide molecular-marker screenings with 119 simple sequence repeats and 560 Diversity Arrays Technology (DArT) markers were employed to identify quantitative trait loci (QTL) for stripe rust resistance. QTL on chromosomes 1AS, 4BL and 7D of ‘USG 3555’ explained 12.8, 73.0 and 13.6% of the variation in stripe rust IT, and 13.5, 72.3 and 10.5% of the variation in stripe rust severity, respectively. Use of these and additional diagnostic markers for these QTL will facilitate the introgression of this source of stripe rust resistance into SRW wheat lines via marker-assisted selection.

Technical Abstract: Little is known about the extent or diversity of resistance in soft red winter wheat (Triticum aestivum L.) to stripe rust, caused by the fungal pathogen Puccinia striiformis f.sp. tritici. The soft red winter (SRW) wheat cultivar ‘USG 3555’ has effective adult-plant resistance to stripe rust, which was characterized in a population derived from ‘USG 3555’/‘Neuse’. The mapping population consisted of 99 recombinant inbred lines, which were evaluated for stripe rust infection type (IT) and severity to race PST-100 in field trials in North Carolina in 2010 and 2011. Genome-wide molecular-marker screenings with 119 simple sequence repeats and 560 Diversity Arrays Technology (DArT) markers were employed to identify quantitative trait loci (QTL) for stripe rust resistance. QTL on chromosomes 1AS, 4BL and 7D of ‘USG 3555’ explained 12.8, 73.0 and 13.6% of the variation in stripe rust IT, and 13.5, 72.3 and 10.5% of the variation in stripe rust severity, respectively. Use of these and additional diagnostic markers for these QTL will facilitate the introgression of this source of stripe rust resistance into SRW wheat lines via marker-assisted selection.