Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/5/2013
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Small ruminants are important agricultural species worldwide; however, diagnostics and research of small ruminant infectious diseases typically rely on cattle-based reagents. One example of this is the lack of small ruminant-derived cell lines to diagnose and study small ruminant viruses. Furthermore, those cell lines that do exist are often not pathologically relevant, or are primary cells. Myeloid-derived monocytes (MDMs) are one example of a cell type that is pathologically relevant to many viruses. MDMs play a key role in the pathogenesis of many viral infections, often in the early steps shortly after the virus penetrates the epithelial barrier. The goal was to create an immortalized, small ruminant-derived MDM cell line permissive to a diverse range of viruses that infect small ruminants. Sheep microglia, which are MDMs, were immortalized by transgenic expression of human telomerase (hTERT). Permissive of the hTERT sheep microglial cells to orbiviruses, pestiviruses, lentiviruses, alphaherpesviruses, and pneumoviruses was demonstrated by the presence of viral antigen, genetic material, and viral infectivity.