|Coyne, Clarice - Clare|
Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2013
Publication Date: 9/4/2013
Citation: Kwon, S.J., Smykal, P., Hu, J., Wang, M., Kim, S., McGee, R.J., McPhee, K., Coyne, C.J. 2013. User-friendly markers linked to Fusarium wilt race 1 resistance Fw gene for marker-assisted selection in pea. Plant Breeding. doi:10.111/pbr.12085. Interpretive Summary: Pea (Pisum sativum L., 2n=14) is an important grain legume crop that has worldwide economic importance as a source of protein for animal and human nutrition. Pea is one of the most important temperate pulse, fodder and vegetable crops. It currently ranks second only to common bean as the most widely grown grain legume in the world with primary production in temperate regions and global production of 10.4 M tons. Fusarium wilt, caused by Fusarium oxysporum Schlecht. f.sp. pisi (Fop) (C.J.J. Hall) Snyder and Hansen (Brayford 1996), is one of the most widespread diseases of pea, limiting pea production throughout the world. Single genes are available for Fusarium wilt resistance, such as Fw for resistance to race 1. Here we report the development of SCAR marker(s) for Fop race 1 resistance in pea using the target region amplification polymorphism (TRAP) marker technique and the consensus components of the plant R-gene sequences as the fixed primers. This work demonstrates the successful application of the TRAP methodology to develop SCAR markers for disease resistance and indicates that these markers and procedures can be readily applied to pea marker assisted breeding programs
Technical Abstract: Fusarium wilt is one of the most widespread diseases of pea. Resistance to Fusarium wilt race 1 was reported as a single gene, Fw, located on linkage group III. The previously reported AFLP and RAPD markers linked to Fw have limited usage in marker-assisted selection due to their map distance and linkage phase. Using 80 F8 recombinant inbred lines (RILs) derived from the cross of Green Arrow × PI 179449; we amplified 72 polymorphic markers between resistant and susceptible lines with the target region amplified polymorphism (TRAP) technique. Marker-trait association analysis revealed a significant association. Five candidate markers were identified, and three were converted into user-friendly dominant SCAR markers. Forty-eight pea cultivars with known resistant or susceptible phenotypes to Fusarium wilt race 1 verified the marker-trait association. These three markers, Fw_Trap_480, Fw_Trap_340 and Fw_Trap_220 ', are tightly linked to and only 1.2 cM away from the Fw locus and are therefore ideal for marker-assisted selection. These newly identified markers are useful to assist in the isolation ofin isolating the Fusarium wilt race 1 resistance gene in pea.