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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #295236

Research Project: Molecular Biology of Human Pathogens Associated with Food

Location: Produce Safety and Microbiology Research

Title: Description of Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

item Fitzgerald, Collette
item Tu, Zheng-chao
item Patrick, Mary
item Stiles, Tracy
item Lawson, Andrew
item Santovenia, Monica
item Gilbert, Maarten
item Van Bergen, Marcel
item Joyce, Kevin
item Pruckler, Janet
item Stroika, Steven
item Duim, Brigitta
item Miller, William - Bill
item Loparev, Vladimir
item Sinnige, Jan
item Fields, Patricia
item Tauxe, Robert
item Blaser, Martin
item Wagenaar, Jaap

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/2/2014
Publication Date: 6/4/2014
Citation: Fitzgerald, C., Tu, Z., Patrick, M., Stiles, T., Lawson, A.J., Santovenia, M., Gilbert, M., Van Bergen, M., Joyce, K., Pruckler, J., Stroika, S., Duim, B., Miller, W.G., Loparev, V., Sinnige, J.C., Fields, P.I., Tauxe, R., Blaser, M.J., Wagenaar, J.A. 2014. Description of Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles. International Journal of Systematic and Evolutionary Microbiology. 64(9):2944-2948.

Interpretive Summary: The food-borne pathogen Campylobacter is a natural contaminant of most birds and livestock. Campylobacters are generally restricted to warm-blooded animals, unlike other pathogens that can grow in the environment or on plants. However, a small subset of campylobacters, highly related to the livestock abortifacient species Campylobacter fetus, are commonly isolated from cold-blooded reptiles, for example snakes, iguanas and pet turtles. These reptile-derived campylobacters can cause illness in humans, presumably through contact and handling of reptiles, although transmission through consumption of reptile meat cannot be ruled out. This study characterizes a reptile Campylobacter strain through genome sequencing, and multiple phenotypic and molecular biological analyses. Results indicate that this strain is an example of a new Campylobacter species, related to C. fetus but distinct with usual (non C. fetus) Campylobacter membrane properties. Analysis of the gene content of this strain will hopefully reveal why this organism can grow and thrive in cold blooded animals and reveal also potential virulence mechanisms that are responsible for disease in humans.

Technical Abstract: A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like isolates from humans (n=8) and reptiles (n=5). Phenotypic characterization, Genusgenus-specific and sap insertion-PCR initially identified all human isolates as type A Campylobacter fetus. Phylogenetic analyseis based on 16S rRNA or hsp60 (groEL) gene sequences revealed that these isolates from humans, along with the isolates from reptiles, formed a robust cluster distinct from the two known subspecies of Campylobacter fetus and other Campylobacter species. Further characterization by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), pulsed- field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) analysis , whole genome sequencing and DNA-DNA hybridization confirmed this divergence. This unique cluster of 13 isolates represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427T (=ATCCxxxxT = LMGxxxxxT) as the type strain.