Location: Warmwater Aquaculture Research UnitTitle: Genetic analysis and antimicrobial susceptibility of Francisella noatunensis subsp. orientalis (sun. F. asiatica) isolates from fish Author
Submitted to: Veterinary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/26/2011
Publication Date: 1/27/2012
Citation: Soto, E., Griffin, M., Wiles, J., Hawke, J. 2012. Genetic analysis and antimicrobial susceptibility of Francisella noatunensis subsp. orientalis (sun. F. asiatica) isolates from fish. Veterinary Microbiology. 154:407-412. Interpretive Summary: Three methods were utilize to determine the antimicrobial susceptibility of 10 different isolates of Francisella noatunensis subsp. Orientalis from two different species and four different geographical outbreaks. Data from this project will be useful as baseline data for monitoring for development of antibiotic resistance for this bacteria and also for development of potential therapeutics.
Technical Abstract: Francisella noatunensis subsp. orientalis (syn. F. asiatica) (Fno) is an emergent fish pathogen that causes acute to chronic disease in a wide variety of freshwater, brackish and marine fish. Due to the emergent nature of this bacterium, established protocols to measure antimicrobial susceptibility are lacking. In this project we compare three different methods to examine the antimicrobial susceptibility (Etest, brothmicrodilution and disk diffusion) of 10 different isolates of Fno from two different fish species and four different geographic outbreaks from 2006 to 2010. PCR mediated genomic fingerprinting (rep- PCR) performed on the different isolates confirmed genetic homogeneity amongst the different isolates. The in vitro susceptibility data presented here provides important baseline data for future research monitoring the development of antibiotic resistance among Fno isolates as well as provides invaluable data for the development of potential therapeutics.