Submitted to: Plant Breeding
Publication Type: Peer reviewed journal
Publication Acceptance Date: 4/11/2013
Publication Date: 6/7/2013
Citation: Ma, Y., Bao, S., Yang, T., Hu, J., Quan, J., He, Y., Wang, X., Wan, Y., Sun, X., Jiang, J., Gong, C., Zong, X. 2013. Genetic linkage map of Chinese native variety faba bean (Vicia faba L.) based on simple sequence repeat markers. Plant Breeding. doi.10.11/pbr.12074. Interpretive Summary: Faba bean (Vicia faba L.) is one of the most important legume crops and provides an important source of protein and starch for human diets and animal feeds in many countries. Being able to fix atmosphere nitrogen and improve soil fertility, faba bean is considered as an essential crop in the sustainable cropping system. Faba bean has an indispensable role in organic agricultural production as a vegetable and a cover crop. SSR (simple sequence repeat) markers are the markers of choice in marker-assisted selection to expedite crop genetic improvement. The available SSR markers for faba bean are limited. This paper reports the construction of a genetic linkage map with both previously reported and newly identified SSR markers using a segregating population derived from two Chinese faba bean varieties. The resulting map and marker information are useful for the faba bean research community.
Technical Abstract: Simple sequence repeat (SSR) marker is a powerful tool for construction of genetic linkage map which can be applied for locating quantitative trait loci (QTL) and marker-assisted selection (MAS). In this study, a genetic map of faba bean was constructed with SSR markers using a population of 129 F2 individuals derived from the cross of Chinese native varieties 91825 (large seed) and K1563 (small seed). By screening 11,551 SSR primer pairs between two parents, 149 primer pairs were detected polymorphic and used for F2 population analysis. This SSR-based genetic linkage map consisted of 15 linkage groups with 128 SSR markers. The map encompassed 1,587 cM with an average genetic distance of 12.4 cM between adjacent markers. The genetic map generated in this study will be beneficial for genetic studies of faba bean for identification of marker-locus-trait associations as well as comparative mapping among faba bean, pea and grasspea.